种子引发ppt22课件.pptVIP

This study was carried out at the Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Iran. Methods ●Control：distilled water ●Osmopriming treatmen：Bromus seeds were immersed in -0.6 MPa of PEG solutions at 25°C for 12h under dark conditions. ●Hydropriming：B. inermis and B. tomentellus seeds were soaked for 8h and 4h in distilled water respectly. ●Drought condition ：PEG 6000 ●Salinity condition ：NaCl solutions ●osmotic potentials：0.3, 0.6, 0.9 and 1.2MPa PEG 6000 Inert, non-ionic and virtually impermeable chains Small enough to influence the osmotic potential, but large enough to not be absorbed by plants . PEG does not enter the apoplast, water is withdrawn from the cell and the cell wall. PEG solutions mimicdry soil more closely than solutions of low osmotic Four replications of 25 seeds were germinated within 90mm Petri dishes and 5ml of salt and drought solutions. Petri dishes and seeds were put into sealed plastic bags to avoid moisture loss. Seeds were allowed to germinate 25±1°C under dark condition for 14 days. Germination was considered to have occurred when radicles were 2mm long. Germination percentage was recorded every 24 h for 14 days. Rate of germination was calculated to assess the speed of germination. Fig. 1 Germination percentage of B. tomentellusa) effects of seed priming treatments on seed germination Fig. 1 Germination percentage of B. tomentellusb) germination percentage at different stress conditions Fig. 2 Effects of seed priming treatments on speed of germination under salinity and drought stresses Fig. 3 Effects of seed priming treatments on root length of B. tomentellus under salinity and drought stress. Fig. 4. a) Effects of seed priming treatments on germination percentage of B. inermis Fig. 4. b) Effects of different stress conditions ongermination percentage of B. inermis Fig. 5 Effects of seed priming on speed of germination of B. inermis at stress conditions Fig