卫气虚模型大鼠血浆代谢标志物月节律变化的研究.docVIP

卫气虚模型大鼠血浆代谢标志物月节律变化的研究 　　摘要：目的 运用代谢组学方法研究卫气虚证月周期性变化的物质基础，寻找其潜在标志物，为卫气虚证实质研究提供新的途径。方法 以秋分日所在的农历月，确定月初（农历八月初一）、月中（八月十五）及月末（八月三十）3次实验的取材时间。取材日前2周将20只大鼠随机分为正常组与模型组，每组10只。模型组采用疲劳联合寒热交替法制备卫气虚模型，正常组常规喂养。3次实验均在其相应取材日正午12：00断头取血，采用高效液相色谱-质谱联用仪检测血浆代谢物，利用偏最小二乘法对数据进行统计分析，组间进行比较，探索卫气虚证差异性代谢标志物，进而推测寻找卫气虚证月周期变化的潜在标志物。结果 油酸酰胺、磷脂酰甘油、皮质醇、脯氨酸、富马酸二甲酯、二十碳五烯酸6种差异代谢物可能为月初卫气虚证潜在证候标志物；1-磷酸鞘氨醇、苹果酸、皮质醇、油酸酰胺、肉毒碱、二十碳五烯酸、富马酸二甲酯7种差异代谢物可能为月中卫气虚证潜在证候标志物；胆固醇乙酸酯、苏氨酸、皮质醇、富马酸二甲酯、油酸酰胺、二十碳五烯酸、焦谷氨酸7种差异代谢物可能为月末卫气虚证潜在证候标志物。结论 卫气虚证月周期性变化可能受油酸酰胺、皮质醇、二十碳五烯酸、富马酸二甲酯影响，其周期性变化可能与能量代谢密切相关，同时还伴随着细胞、激素及神经调节。 　　关键词：卫气虚证；月节律；代谢组学；实验研究 　　DOI：10.3969/j.issn.1005-5304.2016.11.012 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）11-0046-05 　　Abstract： Objective To use metabonomics method to study the change of the basic materials of month rhythm of wei qi deficiency syndrome； To find the potential markers so as to provides a new way for the essence of the wei qi deficiency syndrome research. Methods Based on the autumnal equinox in lunar calendar month， the beginning of a month （the first day of lunar August）， the middle of a month （the 15th day of lunar August）， and the end of a month （the 30th day of lunar August） were set as the three days to draw experimental materials. Two weeks before drawing materials， 20 rats were randomly divided into control group and model group， 10 rats in each group. The model rats were modeled by the stimulus of fatigue combined with coldness and hotness. Control group rats received conventional breeding. The rats in the both groups during the three experiments received decollation and the blood was taken at the 12 o’clock at noon. HPLC-MS was used to detect plasma metabolites， and partial least squares were used to make statistical analysis on the data for comparing plasma metabonomics original data of control group and model group. Possible metabolic markers of wei qi deficiency syndrome were explored， and the potential makers of month rhythm change of wei qi deficiency syndrome were ded