大承气汤对过敏性哮喘小鼠肺组织形态及IgE水平的影响.docVIP

大承气汤对过敏性哮喘小鼠肺组织形态及IgE水平的影响 　　摘要：目的 观察大承气汤对过敏性哮喘小鼠肺组织形态学变化、肺指数、脾指数以及总IgE水平的影响，探讨其相关作用机制。方法 采用卵白蛋白致敏加激发的方式建立小鼠过敏性哮喘模型。将20只雌性C57BL/6小鼠随机分为正常组、模型组、大承气汤组和阳性药组，各给药组给予相应药物灌胃。测定各组小鼠肺指数、脾指数，ELISA测定血清IgE含量，HE染色进行病理观察。结果 与正常组比较，模型组小鼠肺指数增加，血清IgE含量明显升高（P0.01），肺组织出现炎性病理改变；与模型组比较，大承气汤组和阳性药组小鼠肺指数明显降低（P0.05），血清IgE含量明显降低（P0.01），肺组织形态结构明显改善。结论 大承气汤对哮喘小鼠肺部炎症有抑制作用。 　　关键词：过敏性哮喘；肺肠同治；大承气汤；小鼠 　　DOI：10.3969/j.issn.1005-5304.2016.07.015 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0058-03 　　Effects of Dachengqi Decoction on Pulmonary Histology and Total IgE Levels in Mice with Allergic Asthma ZHONG Da-ling， WU Jia-jia， LI Gen-mao， GE Dong-yu， WU Jun， PENG Gui-ying （School of Basic Medical Sciences， Beijing University of Chinese Medicine， Beijing 100029， China） 　　Abstract： Objective To explore the effects of Dachengqi Decoction on morphological changes， lung and spleen indexes， and total IgE levels of mice with allergic asthma； To discuss it relevant mechanism of action. Methods The method of OVA sensitization and provocation was used to establish allergic asthma mice models. 20 C57BL/6 mice were randomly divided into control group， model group， Dachengqi Decoction group and dexamethasone positive group. Each medication group was given relevant medicine for gavage. Lung and spleen indexes in each group were detected. The total IgE levels in serum of mice were detected by ELISA， and HE staining was used to conduct pathological observation. Results Compared with control group， the lung index and level of IgE in model group increased significantly and pulmonary histological results showed abundant inflammatory cells infiltrated in the bronchus； compared with the model group， the lung index as well as the levels of IgE decreased significantly in the Dachengqi Decoction and dexamethasone positive group （P0.05）， and the lung tissue organization form was improved significantly. Conclusion Dachengqi Decoction has inhibiting effects on pulmonary inflammation of mice with asthma. 　　Key word