美国FDA肝钠素物种来源检测方法.docxVIP

开发一种多重实时荧光定量PCR检测法检测 原料中反刍动物DNA监测肝素钠粗品质量深圳市倍素特科技有限公司?作者Sharla M. Peters1, Yolanda L. Jones1, Frank Perrella2, Tai Ha3, and Haile F. Yancy1?1U. S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, Maryland 20708, USA.?2U.S. Food and Drug Administration, Center for Drug Evaluation and Research, Office of Compliance, Silver Spring, MD 20993, USA.?3Nebraska Department of Agriculture, 301 Centennial Mall South, Lincoln, Nebraska 68508, USA.?摘要开发一种实时荧光定量PCR技术检测猪源肝素钠粗品中的反刍动物源污染。该检测法由一套针对反刍动物（牛、绵羊、山羊）和猪的冻干引物、探针及内参组成。该方法经过两处分析机构验证：第一个位于FDA，第二个