indexpagenumber.docVIP

Index Page Number Location of Chemicals 2 How to sharpen microtome blade 3 How to take a picture 4 M1V1=M2V2 (Dilution) Formula 5 How to make solutions 6 A. How to make Mayer’s Albumen Fixative B. How to make Bouin’s Fixative (yellow) C. How to make Eosin Y (1% w/v) Decalcification 7 Liquid for burner 7 Extractomg zebrafish brain 8 Saggital sectioning for IN SITU 9 Fixation and Embedding 10-14 Basic Staining Procedure 15-16 Planes of Section 17-20 Location of chemicals used for Histology ddH2O – 2nd floor EDTA - ? Ethanol (absolute/95%) – 3rd / 4th floor Eosin Y powder – 4th floor histology lab Formalin (10%) – 3rd floor in fridge Formalin/formaldehyde 37% w/w – 3rd floor under hood Glacial acetic acid – 3rd floor under hood Hematoxylin – 4th floor in cabinet next to the sink Methyl Salicylate – 4th floor in cabinet next to the sink Milli-Q water – 3rd floor (use room next to comp. lab) Make sure that the UV light is turn off and regular light is turn on before you enter, when leaving, turn on UV light and turn regular light off, unless someone is there already Also can be found in N411 in Dr. Georgakilas’ Lab, but use it only when it is an emergency Merthiolate - ? Picric acid – 3rd floor under hood RNase away solution - ? Sodium azide - ? Sodium salicylate - ? Thymol - ? Xylene – 3rd / 4th floor How to sharpen microtome blade Get a flat glass from drawer “Block Prep” and place in the proper location in the sharpener and lock it by twisting the knob. Get a blade from the fridge and make sure that it is clean and insert in proper location and lock it by tightening the knob to lock it. From “Centrifuge Tube” drawer, grab 1 coarse abrasive (yellow) bottle and 1 fine abrasive (white) bottle. Shake both bottles well before use. Pour a good amount of coarse abrasive liquid onto the flat glass. Put the switch onto Coarse (blue) and hit the “coarse” button and let it on for 1 hour. Once finish, take the blade out carefully; also t