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Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15–25 years).pdf

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Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15–25 years).pdf

Journal of Immunological Methods 432 (2016) 65–71 Contents lists available at ScienceDirect Journal of Immunological Methods journal homepage: /locate/jim Research paper Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15–25 years) Lavanya Joshi a, Meenakshi Ponnana a, Ramya Sivangala a, Lakshmi Kiran Chelluri b, Pratibha Nallari c, Vijaya Lakshmi Valluri a,d, Sumanlatha Gaddam a,c,? a Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, India b Department of Transplant Biology Stem Cell, Global Hospital Hyderabad, Telangana, India c Department of Genetics, Osmania University, Hyderabad, Telangana, India d LEPRA India, Blue Peter Public Health Research Centre, Cherlapally, Hyderabad, Telangana, India article info Article history: Received 27 November 2015 Received in revised form 9 February 2016 Accepted 9 February 2016 Available online 12 February 2016 Keywords: Household contacts r32kd M. bovis BCG Cytokines Mycobacterium tuberculosis abstract Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any signi?cant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold 1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ IL-6 and sex signi?cantly predicted the HHC group from HCs at p b 0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals. ? 20