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Proteomics analysis of dendritic cell activation by contact allergens reveals possible biomarkers regulated by Nrf2.pdf

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Proteomics analysis of dendritic cell activation by contact allergens reveals possible biomarkers regulated by Nrf2.pdf

Toxicology and Applied Pharmacology 313 (2016) 170–179 Contents lists available at ScienceDirect Toxicology and Applied Pharmacology journal homepage: /locate/taap Proteomics analysis of dendritic cell activation by contact allergens reveals possible biomarkers regulated by Nrf2 Franz Mussotter a,?, Janina Melanie Tomm b, Zeina El Ali c, Marc Pallardy c, Saadia Kerdine-R?mer c, Mario G?tz a, Martin von Bergen b,d,e, Andrea Haase a,1, Andreas Luch a,1 a German Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Berlin, Germany b Helmholtz Centre for Environmental Research (UFZ), Department of Molecular Systems Biology, Leipzig, Germany c INSERM UMR 996, Univ Paris-Sud, Université Paris-Saclay, Chátenay-Malabry, France d University of Leipzig, Institute of Biochemistry, Leipzig, Germany e Aalborg University, Department of Chemistry and Bioscience, Aalborg, Denmark article info Article history: Received 10 August 2016 Revised 28 October 2016 Accepted 1 November 2016 Available online 3 November 2016 Keywords: Allergic contact dermatitis Sensitization Dendritic cell Keap1/Nrf2 Proteomics 2D abstract Allergic contact dermatitis is a widespread disease with high clinical relevance affecting approximately 20% of the general population. Typically, contact allergens are low molecular weight electrophilic compounds which can activate the Keap1/Nrf2 pathway. We performed a proteomics study to reveal possible biomarkers for dendritic cell (DC) activation by contact allergens and to further elucidate the role of Keap1/Nrf2 signaling in this process. We used bone marrow derived dendritic cells (BMDCs) of wild-type (nrf2+/+) and Nrf2 knockout (nrf2?/?) mice and studied their response against the model contact sensitizers 2,4-dinitrochlorobenzene (DNCB), cinnamaldehyde (CA) and nickel(II) sulfate by 2-dimensional polyacrylamide gel electrophoresis (2D) in combination with electrospray ionization tandem mass spectrometry (ESI-MS/MS). Sodium dodecyl