胰α—淀粉酶参考品的实验研究.pdfVIP

中国生化药物杂志Chinese Journal )I Biochemical 1)h㈣ c ti s 2(X)2年第23卷第 1期 1 1 胰a一淀粉酶参考品的实验研究 孟 泽，史利宁，李 勇，黄宇峰 (南京军基 南京，总匡跣 垒王医学检验中心，江苏 南京 210002) 摘 要：目的 研究淀将酶剥定用的。一淀粉酶毒考品 方法 从人胰中用硫酸铵沉淀，DEAE—Sephacel、CM伽S 一 CL-6B度Sephadex( 75三步色谱提纯 用特定的基质稀释分装．制成冻干或液体型．分剐存于一2O 理4℃，研 究其有关性质 结果 提地酶比活为 129 2 kU／g蛋白．Km与人血清中淀粉酶一致；不同方法问的偏差与人血清标 奉相似，有比州互换性；根据加速变性试验推算冻干品在一20％、液体型在4℃的年生活半分别为(】．03％、0 37％： 用IFCC法测定冻干型为(t 048±40 9】U／L，液体型沿(1 t98±32．8)U／L：冻干品复淳柏度液体型再Fj水作1：t稀释 后 淀粉醇活力分别为(537±20 9)U／L度(598±19．8jU／I 结论 此品适台作为淀粉酶测定的参考品，但必额进一 步试用和认证 关键词：胰 淀粉酶；参考品 中图分类号：Q566．2；R392—33 文献标识码：^ 文章编号：1005—1678 c 2002}01—001t-04 Experimental researches on reference material(uncertified)of pancreaticⅡ·amylase MENG Ze．SHI 1J—ning．1A Yong、HUANG Yu—feng 0 Department of LaboratoO,Scief~e Ntl呼ng General Hosphal of N嘶tng Command， 川 ． 研ng 2】0002，Chimt) Abstract：Purpose The aim is IfI prepare a stable and intemlethod properties comparable with those of human seruin specimens the refoI~nce materlal(unterrified1 for the detemllnatlon of d—amvla~．Methods The enzyme was purified frum human pancreas by aWt1]t|oBium sulphate precipitalion and ehn-~mtography successlvely on DEAE—Sephaeel，CM·Sephavose CI一6B and Sephadex( 75，The purified{~nzytlle was diluted Ih a well— defined matrix，dispensing in vials，lyophilizing or continuing liquid form Results The purified enzyme had a specific activity of 129．2 kU／g protein．Only 1L of ALP and 1U of ALT／7850 U of Amy were detected．The material showed intennethod biases similar to these for human seillIn．The yearly loss of a( tivitY f r thc DI 口a— ration of lyophilized and liquid form on the basis of accelerated degradation studies were 0．03％ at一20~C． and 0．37％ a