D-Dimer检测的相关话题选编.pptx

D-Dimer 检测的相关话题 希森美康医用电子（上海）有限公司 学术应用课 凌励 话题列表 D-Dimer的相关概念 D-Dimer检测方法概述 INNOVANCE D-Dimer 试剂介绍 D-Dimer检测的临床意义 D-Dimer检测的误差分析 话题列表 D-Dimer的相关概念 D-Dimer检测方法概述 INNOVANCE D-Dimer 试剂介绍 D-Dimer检测的临床意义 D-Dimer检测的误差分析 Thrombin Factor XIII Plasmin 8 FDPs VS D-Dimer: 生理学 FDPs FDPs 实验测定纤维蛋白(原)总体降解产物 预示血管内外大量伴或不伴有凝固活化的纤溶发生 D-Dimer D-Dimer 实验测定交联纤维蛋白降解产物 预示由凝固信息诱使的继发性或反应性纤溶 9 FDPs VS D-Dimer: 病理学 FDPs 纤溶活化的任何阶体现了机体纤溶激活水平 体现机体纤溶活化物与抑制物的平衡状态 体现了一定基础上的纤溶总体活性水平 D-Dimer 继发在凝血酶生成之后的纤溶 体现任何形式的凝血酶或血栓形成 体现在各种原因引起血管内皮损伤 体现在自身或题外因素导致的溶栓过程,与体内纤溶能力无直接关系 10 话题列表 D-Dimer的相关概念 D-Dimer检测方法概述 INNOVANCE D-Dimer 试剂介绍 D-Dimer检测的临床意义 D-Dimer检测的误差分析 D-dimer laboratory assays Currently available D-dimer assays are not identical D-dimer antigen is present on different size degradation products, the monoclonal antibodies recognize different epitopes, the assay format, assay calibration standards, instrumentation vary. Standardization of D-dimer Assays International Society of Thrombosis and Hemostasis Scientific Standardization Subcommittee Efforts to standardize assay results have not been successful so far. Because the D-dimer analyte is not uniform across the different assays. Accordingly, there were attempts to harmonize assay performance through the interconversion of results from different assays using specific mathematical formulas,but this has yet to be accepted as universal practice. 第一代血浆D-Dimer检测方法 原理：Using latex beads coated with the DD-3B6 antibody. Latex agglutination assays rely upon the presence of sufficient bivalent D-dimer antigen on fibrin degradation products to initiate agglutination. Early assays required laboratory personnel to visually read and report the magnitude of the agglutination response 14 Greenberg CS, Devine DV, McCrae KM. Measurement of plasma fibrin D-dimer levels with the use of a monoclonal antibody coupled to latex beads. Am J Clin Pathol. 1987;87:94-100. Automated latex agglutination assays 原理：That recorded the rate at which antibody-coate