全基因组重测序揭示了家鸡驯化过程中受选择的位点讲解.pptxVIP

文献汇报 汇报人: 付玮玮 2016年7月8日 文章框架 SOLiD Resequencing Red jungle fowl, broiler, layer (8 populations) SNP detection and analysis. Selective-sweep analysis (Hp/ZHp) Detection of deletions and stop codons Validation of loss-of-function mutations 研究结果 8个家鸡群体和1个红原鸡群体的重测序 （4-5 X） n = 8 n = 10 n = 10 n = 11 n = 11 n = 8 n = 10 n = 11 n = 8 Figure 1 | Chicken lines resequenced. 三个独立的reads上均具有相同的non-reference nucleotide被认为是一个多态位点; 7,453,845 SNP loci remained after this filtering Figure 2 | Selective-sweep analysis of the chicken genome. Hp value of 0.09 (ZHp = -8.2) over BCDO2 8个家鸡群体 WL-A WL-B RIR CB-1 CB-2 受选择的基因 特征 ZHPf HP ADg HP CBg HP LRg 染色体 BCDO2 黄色皮肤 AD：-8.3 0.09 0.14 0.14 24 SEMA3A 脑信号蛋白 AD 1 VSTM2A 膜融合、神经（target-SNARE gene） AD：-7.7 0.11 0.17 0.13 2 TSHR 代谢调控和生殖 AD：-9.2 LR：-4.9 CB：-4.7 0.06 0.06 0.06 0.09 0.09 0.09 0.07 0.07 0.07 5 IGF1 生长 LR：-5.6 CB：-6.6 0.28 0.29 0.14 0 0.04 0.14 1 PMCH 食欲和代谢调控 CB：-6.6 0.29 0 0.14 1 TBC1D1 located major QTL explaining differences in growth between broilers and layers in three independent studies CB：-6.7 0.29 0 0.31 4 Figure 2 | Selective-sweep analysis of the chicken genome. 0.1% n=58 0.05% 0.01% Figure 3 | Details of TSHR mutation and sweep region. Gly588Arg GHR: a causative mutation for sex-linked dwarfism and has been used in some commercial broiler lines; SH3RF2: lies within a QTL region for body weight detected in a cross between the high and low growth lines; deletion is fixed in the high growth line 1,300 deletions that were fixed or close to fixation in at least one population Figure 4 | Characterization of the SH3RF2 deletion. 谢谢！ 感 谢