氟影响大鼠牙釉质发育的扫描电镜和原子力显微镜观察.pdfVIP

史垄口腔医擎杂志 2015 年5 月第 50 卷第5 期 Chin .T Stomatol. Mav 2015. Vol. 50. Nι5 , 307 ·牙体牙髓病学研究· 氟影响大鼠牙袖质发育的扫描电镜 和原子力显微镜观察 陈黎明汤畔唐杰 [摘要] 目的 观察大鼠氟斑牙模型下颁切牙袖质的超微结构，探讨过量氟对牙体微观结构的 影响。方法 30 只5 周龄50 大鼠雌雄各半，均分为3 组，对照组用不含氟的去离子水喂养，低氟组用 含22.5 mglL V(50 mgIL NaF) 、高氟组用含45 mglL F-( 100 mglL NaF)的去离子水喂养;饲养8 周后，取 所有大鼠下顿切牙，制备用于扫描电镜和原子力显微镜(atomic force microscopy ,AFM )观察的牙体组 织切片，分析比较不同氟浓度对大鼠袖质微观结构的影响。结果 扫描电镜结果显示，对照组样本袖 质中袖柱结构完整，晶体排列紧密，层次清晰;高氟组样本和柱结构瓦解，晶体松散、弯曲折断;低氟组 样本袖柱结构形态的变化介于对照组与高氟组之间。 AFM结果显示，对照组、低氟组及高氟组样本 袖质中层的平均粗糙度分别为(550.6:t32.0) 、 (415.0:t24.2)及(194.3 :tl 1.3) nm，高氟组与对照组相比 差异有统计学意义(P0.05) 。结论 在高氟环境下形成的大鼠牙袖质出现明显的结构拥塌，袖柱间 无间隙;高氟可造成大鼠发育中牙轴质的袖柱晶体正常结构丧失。 [关键词] 氟中毒，牙; 牙袖质; 显微镜检查，原子力; 显微镜检查，电子，扫描 Atomic force microscopy and scanning electron microscopic study on the t1uorosis of enamel in rats Chen μming， Ta咱 Ye， Tang Jie. Department o/Operative Dentistry and Endodont町， Glμyang Hospital 0/ Stomatology, Guiyang 550001, China Corresponding author: Chen Liming, Email: 1518071143αg) 163.com , Tel: ω86-851 [Abstract] Objective To investigate the ultrastructure of enamel in the mandibular incisor tooth in a rat model of dental fluorosis. Methods Thirty 5-week-old male and female rats of 50 strain were divided into three groups of ten. The animals in the control group were maintained for 8 weeks on pure deionized water , in the low-fluoride group deionized water with 22.5 mgIL of fluorine(50 mgIL NaF) was used , and in the high -fluoride 伊咽up deionized water with 45 mgIL of fluorine(l00 mgIL Na F) was used. All specimens examined were obtained from the mandibular incisors. Mid-sagittal ground sections were prepared from the direction of incisal point of each incisor for scanning electron microscopy(5EM) and atomic force microscopy (AFM). Results According to the 5EM resu