基于透射解析模型的时域扩散荧光层析原理与实验.pdfVIP

第 43 卷 第 6 期 2010 年 6 月 天 津 大 学 学 报 Journal of Tianjin University Vol.43 No.6Jun. 2010 收稿日期：2008-07-31；修回日期：2009-09-02. 基金项目：国家自然科学基金资助项目30970775)；国家重点基础研究发展计划 (973 计划)资助项目(2006CB705700)；国家高技术研究发展计划(863 计划)资助项目(2009AA02Z413)；天津市自然科学基金资助项目(09JCZDJC18200). 作者简介：高 峰（1963— ），男，博士，教授，gaofeng@. 通讯作者：李 娇，jo412608922@. ▋ 基于透射解析模型的时域扩散荧光层析原理与实验 高 峰，李 娇，张丽敏，赵会娟 (天津大学精密仪器与光电子工程学院，天津 300072) 摘 要：利用基于耦合扩散方程拉氏变换的广义脉冲谱技术和基于玻恩比的逆模型归一化表示形式，提出了无限平板透射模式下的时域扩散荧光层析图像重建方法．该方法采用外推边界条件下无限平板结构扩散方程的解析解，通过引入一对实拉氏变换因子及相应的两次线性求逆，可实现荧光产率和寿命的同时重建．将提出的算法进行了数值模拟验证，在空间分辨率、噪声鲁棒性等方面进行了评估．通过实验验证获得的荧光参数空间分布较准确的重建结果证明了此重建算法的有效性和可行性． 关键词：扩散荧光层析；扩散方程解析解；代数重建技术；广义脉冲谱技术；时间相关单光子计数 中图分类号：R338.8 文献标志码：A 文章编号：0493-2137(2010)06-0557-05 A Methodological and Experimental Investigation on Time-Domain Fluorescence Diffuse Optical Tomography Based on Analytic Transmission Scheme GAO Feng，LI Jiao，ZHANG Li-min，ZHAO Hui-juan (School of Precision Instrument and Opto-Electronics Engineering，Tianjin University，Tianjin 300072，China) Abstract：An image reconstruction scheme for time-domain fluorescence diffuse optical tomography in transmission mode of a slab turbid geometry has been proposed based on generalized pulse spectrum technique that employs ana- lytic expressions of the Laplace-transformed time-domain photon-diffusion model to construct the Born normalized inverse model. A pair of real domain transform-factors and two linear inverses have been adopted，and analytic solu- tion to the diffuse equation of a slab turbid geometry in extroplation boundary conditions has been utilized to achieve reconstruction of both the fluorescent yield and lifetime. Numerical simulation has been conducted to validate the al- gorithm，and its spatial resolution and noise-robustness have been evaluated. Reconstruction results with accurate spatial distribution of fluorescent factors obtained in experiments have verified effectiveness and feasibility of the al- gorithm proposed. Keywords：fluor