扫描电镜观察组织工程神经支架与骨髓间充质干细胞的共同培养.pdfVIP

q阻四R 中型组织工程研究与ilffil在康复第 Il~章第 20JI/j 2007-05-20 出版Journalof Clinical Rehabilitative Tissue Engine四fngRe5f.坦rch May 20, 2007 VoI.11, NO.20 .L革_11 学 扫描电镜观察组织工程神经支架与骨髓间充质子细胞的共同培养女 去彩JI蜀，吕 同H Department 01 O晴、opaedics， First Affiliat.国 H西pi划， China Medical Universi弘部回，yang 110001 , Uaoning Province, China Zhang Cai-sh泣。会， Doct町AIt宙飞ding physicia吼 Depa措ment 01 Orthopaed峙. Fi陪t Affiliat时 Hos扳倒， Chi阳Medical Unive陪ity， Shenyang 110001, Uaoning Province, China Z臼731009@163∞m Correspondence 10: Lü Gang, Prolessor, Tutor 01 d∞tor， Department 01 O民hopaedics ， First AI贯liated Hospital, China Medical Unive目沟， Shenyang 11∞的Liaoning Province, China Received: 2007毛3-13 Accepte在 2007... 怜16 中国军科大学附属 第一医混骨科，辽宁 省沈阳窜 110001 张彩 J~ 会，军， 1973 年生，辽宁省丹东市 人，汉族，2007 年中 国医科大学毕泣，博 士，主治运师，主要 从事周酒神经组织 工程方边的研究。 zcs731随@163剧n 通讯作者:吕 尉， 教授，博士生导师， 中国医科大学黯属 第一医院骨科，辽宁 吝沈阳亨刊号001 申理分类号:R31B 立戴棕误码 B 文章编号:1673-8225 (2007)2饲3部8-03 b在鸪日美军，2007-O~弘13 啻回日黯，2007毛牛16 幸)7-50-3怀1457.GW.Y) 3988 Culture of bone marrow stromal cells on tissue engineered nerve scaffold evaluated by scanning electron rnicroscope Abstract AIM:τo study Ihe morphology of bone marrow slromal 出IIs (BMSCs) ∞Ilur创 on tissue engineered nerve sca汗。Id using s臼nning eleclron micros∞py (SEM), and investigate Ihe affinily of BMSCs to nerve s臼ffold MEmoDS:τhe expe时ment was conducled at Ihe Neuroanatomy Laboratorγof China Medical University from February 10 December in 2006. Adult male Wistar rats of cleaning grade, w?ighing 180-200 g, we陀 used to prepare acellular nerve 9陪我， while those weighing 100-120 9 were used for BMSCs. AII the animals were offered by the Experimental Animal Departmenl of China Medical υniversity (No.SCXK2003-0009).( Acellular nerve gra传 was prepared according to Liu et als method for tissue engineered nerve s臼仔Old.②In vítro cullure and idenlificalion of BMSCs:τhe femur and tibia were separaled from Wistar rals under ste时le ∞ndition. 在lIedullary canal was repealedly rinsed with low-glucose DMξM sol