益生菌衍生的可溶性因子对于宿主防御的调节机制：对消化系统的健康影响综述.ppt

Sometimes your title for the slide is in yellow font sometimes in white, should pick one be consistent. Fang, not sure I understand this model. It looks like you are showing p40 binding the EGFR. Don’t you want the arrow to be pointed towards the cell with a “?” mark? * * Will be important to walk them through experiment results. * * * Fang, are you going to animate the box appearance since it is hard to see what the boxes are trying to show. If you animate them in a paired way that you plan to discuss them it will focus attention. Also, if the ADAM drawing is from a manuscript you should include the reference * * Note blue font in title: Do you know that activity is required or do you just know that TACE expression is required? I don’t see data showing MMP-inactive TACE. Blue font is just so you will know the wording changes. * * * Summary p40-stimulated TACE activity, leading to HB-EGF release, serves as a mechanism underlying EGF receptor transactivation in intestinal epithelial cells 在肠上皮细胞，P40-刺激TACE的活性，导致HB-EGF释放，从而使其在表皮生长因子受体下转录 Aim 3 To test the hypothesis that p40 prevents experimental colitis in mice in an EGFR-dependent manner. 为了检验这个假设，p40依赖表皮生长因子受体来抑制小鼠的实验性结肠炎 Preparation of pectin/zein beads for specific delivery of p40 to the colon in vivo. 制备果胶/玉米醇溶蛋白的药丸（包埋有p40），能够使其在体内到达结肠位置。 Gavaged with pectin/zein beads (2 beads/mouse) containing FITC-His-p40 or His-p40 - Sacrificed after 4 hours. FITC-labeled His-p40 beads His-p40 beads Green: FITC-His-p40 Red: E-cadherin Blue: DAPI Stomach Small intestine Colon Colon pectin/zein bead His-p40: 5 ?g/bead 1 cm Yan et al., JCI, 121, 2242, 2011 -灌胃果胶/玉米醇溶蛋白的药丸（每只小鼠两颗），药丸里面包含有FITC-His-p40（标记的）或His-p40（未标记的） -药丸在4h后完全消化 上表皮钙粘着蛋白 p40 activates EGFR and Akt in colon epithelial cells in vivo. 在体内的结肠上皮细胞中，p40激活EGFR和Akt Yan et al., JCI, 121, 2242, 2011 磷酸化表皮生长因子受体 p40 prevents DSS-induced colitis in wt, but not EGFRwa2 mice. 在wt小鼠