青蒿素和青蒿琥酯对人乳腺癌MCF—7细胞的体外抑制作用比较研究.pdfVIP

中草药 Chinese Traditional and Herbal Drugs 第 34卷第 4期 2003年 4月 ·347· 青蒿素和青蒿琥酯对人乳腺癌 MCF一7细胞的体外抑制作用比较研究 林 芳 ，钱之玉 ，薛红卫 ，丁 健。，林莉萍。 (1．中国药科大学 药理教研室，江苏 南京 210009；2．中国科学院上海植物生理生态研究所 分子遗传国家重点实验室，上 海 200032；3．中国科学院上海药物研究所 肿瘤药理组，上海 200032) 摘 要：目的 对比研究青蒿素及其衍生物青蒿琥酯对人乳腺癌 MCF一7细胞增殖的影响并探讨其作用机制。方法 采用体外培养的人乳腺癌 MCF一7细胞株，利用 SRB法测定青蒿素和青蒿琥酯对 MCF一7细胞增殖的影响，FCM 法测定细胞周期的变化，亚G 期含量测定和 DAPI荧光染色法观察细胞凋亡。结果 10~tmol／L青蒿素和 1~tmol／ L青蒿琥酯能明显改变 MCF一7细胞的细胞周期，使 S期细胞显著减少，Go+G 期细胞明显增加。青蒿素对 MCF一 7细胞增殖仅有微弱抑制作用，但其衍生物青蒿琥酯却有显著的抑制作用，IC o为 0．31~tmol／L。同样，1~tmol／L青 蒿琥酯引起 MCF一7细胞的凋亡和直接的细胞毒作用明显强于 10~tmol／L青蒿素的作用。结论 体外研究表明，对 肿瘤细胞增殖的抑制青蒿琥酯比青蒿素作用强。 ’ 关键词：青蒿紊；青蒿琥酯；MCF一7细胞 中圈分类号：R286．91 文献标识码：A 文章编号：0253—2670(2003)04—0347—03 Comparison of inhibitory effects between artemisinin and artemisunate on．proliferation Of M CF一7 cells in v ro LIN Fang ，QIAN Zhi—yu ，XUE Hong—wei ，DING Jian。，LIN Li-ping。 (1．Department of Pharmacology，China Pharmaceutical University，Nanjing 2 10009，China；2．National Laboratory of Plant Molecular Genetics，Shanghai Institute of Plant Physiology，CAS。Shanghai 200032，China； 3．Shanghai Institute of Materia Medica，CAS，Shanghai 200032，China) _ Abstract：Object To study the effects of artemisinin and its analogue artemisunate on the prolifera— tion of human breast cancer M CF一7 cell line．as well as their mechanism Comparatively．Methods The in— hibition of cell proliferation was determined by SRB method．Cell cycle was determined by flow cytometry (FCM)analysis．Apoptosis was confirmed by sub—G cells content and DAPI method．Results The cell cycle of MCF-7 was changed greatly when treated 24 h with either 10 ttmol／L artemisinin or 1 ttmol／L artemisunate，the distribution of MCF一7 cells among S phase was reduced greatly，while inereased during G0+ G1．However，artemisinin had weaker effect on the proliferation．of MCF一7 cell，while artemisunate effectively inhibited the proliferation of MCF一7，the IC50 was 0．3 1 ttmol／L．A poptosis induced by 1~mol／ L artemisunate was stronger than that by 10 ttmol一／L artemisinin，tOO．