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5.Characterization and identification of the candidate gene of rice thermo-sensitive genic male.pdf

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5.Characterization and identification of the candidate gene of rice thermo-sensitive genic male

Planta (2007) 225:321–330 DOI 10.1007/s00425-006-0353-6 ORIGINAL ARTICLE Characterization and identiWcation of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping Qingkai Yang · Chunyang Liang · Wen Zhuang · Jun Li · Huabing Deng · Qiyun Deng · Bin Wang Received: 27 March 2006 / Accepted: 22 June 2006 / Published online: 2 August 2006 ? Springer-Verlag 2006 Abstract Previous research has demonstrated that the thermo-sensitive genic male-sterile (TGMS) gene in rice was regulated by temperature. TGMS rice is important to hybrid rice production because the appli- cation of the TGMS system in two-line breeding is cost-eVective, simple, eYcient and overcomes the limi- tations of the cytoplasmic male sterility (CMS) system. AnnongS is the Wrst discovered and deeply studied TGMS rice line in China. Previous studies have sug- gested that AnnongS-1 and Y58S, two derivative TGMS lines of AnnongS, were both controlled by a single recessive gene named tms5, which was geneti- cally mapped on chromosome 2. In the current study, three populations (AnnongS-1 ￡ Nanjing11, Y58S ￡ Q611, and Y58S ￡ Guanghui122) were developed to investigate the tms5 gene molecular map. Analysis of recombination events of sterile samples, utilizing 125 probes covering the tms5 region, suggested that the tms5 gene was physically mapped to a 19 kb DNA frag- ment between two markers, 4039-1 and 4039-2, located on the BAC clone AP004039. Following the construc- tion of a physical map between the two markers, ONAC023, a member of the NAC (NAM-ATAF- CUC-related) gene family, was identiWed as the candi- date of the tms5 gene. Keywords Physical mapping · Rice · Thermo-sensitive genic male-sterile rice · tms5 gene Abbreviations TGMS Thermo-sensitive genic male-sterile CMS Cytoplasmic male sterility NAC NAM-ATAF-CUC-related Introduction The fertility of thermo-sensitive genic male-sterile (TGMS) rice lines is regulated by environmental tem- perature changes. Consequently, its