ALSV-vector.pdfVIP

Virology 386 (2009) 407–416 Contents lists available at ScienceDirect Virology j ourna l homepage: www.e lsev ie / locate /yv i roApple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes Aki Igarashi a, Kousuke Yamagata a, Tomokazu Sugai a, Yukari Takahashi a, Emiko Sugawara a, Akihiro Tamura a, Hajime Yaegashi b, Noriko Yamagishi a, Tsubasa Takahashi c, Masamichi Isogai a, Hideki Takahashi d, Nobuyuki Yoshikawa a,b,c,? a Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan b The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan c The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550, Japan d Department of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan? Corresponding author. Plant Pathology Laboratory University, Morioka 020-8550, Japan. Fax: +81 196 23 6 E-mail address: yoshikawa@iwate-u.ac.jp (N. Yoshik 0042-6822/$ – see front matter ? 2009 Elsevier Inc. A doi:10.1016/j.virol.2009.01.039a b s t r a c ta r t i c l e i n f oArticle history: Apple latent spherical virus Received 17 November 2008 Returned to author for revision 20 December 2008 Accepted 10 January 2009 Available online 25 February 2009 Keywords: Apple latent spherical virus vector Virus-induced gene silencing Arabidopsis thaliana Tobacco Cucurbit Legume(ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The sil