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Aptamer-Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells.pdf

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Aptamer-Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells

Aptamer-Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells Guodong Liu,*,?,? Xun Mao,? Joseph A. Phillips,§ Hui Xu,? Weihong Tan,*,§ and Lingwen Zeng*,? Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China 510663, Department of Chemistry and Molecular Biology, North Dakota State University, Fargo, North Dakota 58105, and Center for Research at Bio/Nano Interface, Department of Chemistry, Shands Cancer Center, Department of Physiology and Functional Genomics, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200 We report an aptamer-nanoparticle strip biosensor (ANSB) for the rapid, specific, sensitive, and low-cost detection of circulating cancer cells. Known for their high specificity and affinity, aptamers were first selected from live cells by the cell-SELEX (systematic evolution of ligands by exponential enrichment) process. When next combined with the unique optical properties of gold nanoparticles (Au-NPs), ANSBs were prepared on a lateral flow device. Ramos cells were used as a model target cell to demon- strate proof of principle. Under optimal conditions, the ANSB was capable of detecting a minimum of 4000 Ramos cells without instrumentation (visual judgment) and 800 Ramos cells with a portable strip reader within 15 min. Importantly, ANSB has successfully detected Ramos cells in human blood, thus providing a rapid, sensitive, and low-cost quantitative tool for the detection of circulating cancer cells. ANSB therefore shows great promise for in-field and point-of-care cancer diagnosis and therapy. The noninvasive early detection of cancer and monitoring of its progress are high on the agenda of oncologists.1 Studies suggest that the circulating cancer cells found in patients are associated with short survival.2 Cancer of blood including leuke- mia, lymphoma, and myeloma is one of the most common ty