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The impact of particle size and initial solid loading
ORIGINAL PAPER
The impact of particle size and initial solid loading
on thermochemical pretreatment of wheat straw
for improving sugar recovery
Oscar A. Rojas-Rejo?n ? Arturo Sa?nchez
Received: 30 April 2013 / Accepted: 15 December 2013 / Published online: 4 January 2014
Springer-Verlag Berlin Heidelberg 2013
Abstract This work studies the effect of initial solid load
(4–32 %; w/v, DS) and particle size (0.41–50 mm) on
monosaccharide yield of wheat straw subjected to dilute
H2SO4 (0.75 %, v/v) pretreatment and enzymatic sac-
charification. Response surface methodology (RSM) based
on a full factorial design (FFD) was used for the statistical
analysis of pretreatment and enzymatic hydrolysis. The
highest xylose yield obtained during pretreatment (ca.
86 %; of theoretical) was achieved at 4 % (w/v, DS) and
25 mm. The solid fraction obtained from the first set of
experiments was subjected to enzymatic hydrolysis at
constant enzyme dosage (17 FPU/g); statistical analysis
revealed that glucose yield was favored with solids pre-
treated at low initial solid loads and small particle sizes.
Dynamic experiments showed that glucose yield did not
increase after 48 h of enzymatic hydrolysis. Once estab-
lished pretreatment conditions, experiments were carried
out with several initial solid loading (4–24 %; w/v, DS)
and enzyme dosages (5–50 FPU/g). Two straw sizes (0.41
and 50 mm) were used for verification purposes. The
highest glucose yield (ca. 55 %; of theoretical) was
achieved at 4 % (w/v, DS), 0.41 mm and 50 FPU/g. Sta-
tistical analysis of experiments showed that at low enzyme
dosage, particle size had a remarkable effect over glucose
yield and initial solid load was the main factor for glucose
yield.
Keywords Thermochemical pretreatment Wheat
straw Enzymatic saccharification Cellulosic ethanol
Introduction
Lignocellulosic biomass represents the largest source of
materials currently available for production of alternative
biofuels and high value-added bioproducts.
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