冷胁迫处理下冬小麦东农冬麦1号叶片蛋白质的差异表达分析.pdfVIP

第45卷第2期 2014年2月 东北农业大学学报 Journal of Northeast Agricultural University 网络出版时间 2014-1-17 16:43:31 [URL] lkcmsldetaiIl23.1391.S.20 140 117 .1643 .020.h恤I 冷胁迫处理下冬小麦东农冬麦 1 号叶片 蛋白质的差异表达分析 苍晶，韩瑞，于晶，徐庆华，赫福霞，李怀伟，赵浮彤，张克俭 (东北农业大学生命科学学院，晗尔滨 150030 ) 45(2): 1-4 February 2014 摘 要:以冬小麦东农冬麦 1号为试验材料，将三叶期麦苗经4 C冷胁迫处理7d后提取叶片蛋白 (25 c处理为 对照纽)，利用双向电泳(2-DE)技术分析差异表达蛋白。所得双向电泳图谱经ImageMaster 2D Platinum 7软件分析 检测到 26个差异表达蛋白质点，选取其中 3个进行质谱(MALDI-TOF-MS)分析，所得肤段序列经数据库搜索比对 获得的匹配蛋白有3-磷酸甘油路脱氢酶、 ATP酶和Rubisco活化酶。这3种蛋白可能通过提高植物的光合能力、促 进植物体内糖分的积累、改善植物对水合离子吸收等方面参与植物的抗冷机制。 关键词:冬小麦;冷胁迫;双向电泳;差异蛋白 中图分类号: S767.5; XI72 文献标志码:A 文章编号: 1005-9369(2014)02-∞01-04 苍晶，韩瑞，于晶，等.冷胁迫处理下冬小麦东农冬麦 1 号叶片蛋白质的差异表达分析[J). 东北农业大学学报， 2014 ， 45(2): 1-4 Cang Jing, Han Rui, Yu Jing, et al. Analysis of expressed di何erential proteins in leaf of winter wheat Dongnongdongmai 1 under ∞Id stress[J). Joumal of Northeast Agricultural Unive陪ity， 2014, 45(2): 1-4. (in Chinese with English abstract) Analysis of expressed differential proteins in leaf of winter wheat Dongnongdongmai 1 under cold stress/cANG Jing, HAN Rui, YU Jing, XU Qinghua, HE Fuxia, LI Huaiwei, ZHAO Botong, ZHANG Kejian (School of Life Scien?s, Northeast Agricultural University, Harbin 150030, China) Abstract: To剧 proteins from the leaves of winter wheat Dongnongdongmai 1 were e刘racted when seedlings at three-Ieaf p创od treated at 4 oc ∞Id stress for seven days (seedlings treated at 25 oc as ∞n- trol), and diffe陪ntially expressed pro剧ns were analyzed by two-dimensional electrophoresis (2-DE). The 2-DE maps obtained were analyzed by ImageMaster 2D Platinum 7 software and 26 di何erentially expressed protein spots we陪 detected ， 3 spots were selected for MALDI-TOF-MS analysis and peptide fragment se- quen四s were searched in the database which were matched to gly饵阳Idehyde-3-phosphate dehydroge- nase, ATPase and Rubis∞ activase. These three proteins might involved in the mechanisms of ∞Id resis- tan? through enhancing the photosynthetic 臼pacity， promoting the accumulation