甲胎蛋白阴性与甲胎蛋白阳性肝细胞癌基因表达谱差异研究.pdfVIP

中华普通外科杂志 2016 年 5 月第 31 卷第 5 期 Chin J Gen Surg , May 2016 ,Vo1. 31 , No.5 甲胎蛋白阴性与甲胎蛋白阳性肝细胞癌 基因表达谱差异研究 安松林肖 f丁冯林郭宏林荣维洪王黎明吴凡冯莉吴俊雄 [摘要] 目的 肝细胞癌是具有不同临床表现和分子生物学特征的异质性疾病，本研究比较甲 胎蛋白 (alpha-fetoprotein ， AFP) 阴性和 AFP 阳性肝癌的 mRNA 表达谱差异，并进行生物信息学分析 3 方法 采用安捷伦 4 x44k 基因芯片平台，检测合并乙型肝炎病毒( hepat阳 ? virus , HBV) 感染的 2019uAFP 阴性和 20 例 AFP 阳性肝癌组织的 mRNA 表达谱.用生物信息学方法进行差异基因分析及 GO Pathway 分析，并用 real time PCR 验证芯片结果。 结果 通过表达谱芯片分析，在 AFP 阴性和 AFP 阳性肝癌组织中发现 352 个差异表达基因。主成分分析及 GO Pathway 分析显示， AFP 阴性肝癌 组织高表达基因 62 个，主要参与细胞凋亡和程序性死亡等过程，AFP 阳性肝癌高表达基因 290 个，主 要参与有丝分裂和细胞周期等过程。 real time PCR 验证差异基因 LAMB3 和 STAR囚的表达，结果与 表达谱芯片结果一致。 结论 AFP 阴性肝癌和 AFP 阳性肝癌基因表达特征不同，提示不同 AFP 水 平的肝癌的发生和发展存在不同的分子机制。 {关键词] 癌，肝细胞; 甲胎蛋白类 DifTerences in mRNA expression profiles between alpha-fetoprotein negative and positive hepatocellular carcinoma An Songlin , Xiω Ting ， Feng Lin , Guo Honglin , Rοng Weiqi , W，αng Liming , 膨u Fan , Feng μ ， Wu Jianxiong. Department 0/ Oneology Surgery , Be?ing Sl吵Lωn Hospi时 ， Be?ing 100038. China Corresponding author: W u Jianxiong , E-mail: dr.wujx@hotmail. com [Abstract] Objective To comp田e gene expression profiles between AFP negative and AFP pusit川 hcpatoccllular carcinoma (HCC). Methods The analysis of mRNA cxpression was performcd on 20 samples of AFP negative and 20 samples of AFP positive HCC associated with HBV infection through Agilent@ Whole Human Genome Oligo Microarray. Bioinformatics analyses were used to identify genes and real time PCR was applied to verify microarray data. Results 352 differentially expressed genes in the two groups were idenlifieu lhrough principal componenl analysis. 62 up-regulated genes in AFP negalive HCC were found to be involved in cell apoptosis and programmcd cell death , whereas 290 up-regulatcd genes in AFP positive HCC involved in mitosis and cell cycle. Two differentially expressed genes including LAMB3 and STARDS were further tested through real time PCR , and the results were in high concordance with microarray results. Conclusion The