2009年的测试标准.pdf

OECD/OCDE XXX Draft: 15 November 2009 OECD GUIDELINES FOR THE TESTING OF CHEMICALS PROPOSAL FOR A NEW GUIDELINE Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment INTRODUCTION 1. This test guideline is designed to assess the effects of life-long exposure of chemicals to the freshwater dipteran Chironomus sp, fully covering the first generation and the early part of the second generation. It is an extension of the existing OECD test guideline 219 or 218 using a spiked-water exposure scenario (1) or a spiked sediment scenario (15), respectively. It takes into account existing toxicity test protocols for Chironomus riparius and Chironomus dilutus (previously named C. tentans (2)) that have been developed in Europe and North America (3-9) and subsequently ring-tested (1, 7, 10, 11, 12). Other well documented chironomid species may also be used, e.g. Chironomus yoshimatsui (13, 14). 2. Both water and sediment exposure scenarios are described in this guideline. The selection of an appropriate exposure scenario depends on the intended application of the test. The water exposure scenario, spiking of the water column, is intended to simulate a pesticide spray drift event and covers the initial peak concentration in surface waters. Water spiking is also useful for other types of exposure (including chemical spills), but not for accumulation processes within the sediment lasting longer than the test period. In that case, and also when run-off is the main entry route of pesticides into water bodies, a spiked sediment design may be more appropriate. If other exposure scenarios are of interest, the test design may be readily adapted. For example, if the distribution of the test compound between the water phase and the sediment layer is not of interest, and adsorption to the sediment has to be minimized the use of a surrogate artificial sediment (e.g. quartzsand) may be considered. 3. Substances