bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro.pdf

Biomaterials 26 (2005) 303–310 ARTICLE IN PRESS*Correspondin cal and Biomedi University of Mi Ann Arbor, MI 4 2110. E-mail addres 0142-9612/$ - see doi:10.1016/j.bioEffects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro William L. Murphya, Susan Hsiongb, Thomas P. Richardsona,c, Craig A. Simmonsa,c, David J. Mooneya,b,c,* aDepartments of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA bDepartment of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA cDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA Received 30 September 2003; accepted 9 February 2004Abstract Multipotent cell types are rapidly becoming key components in a variety of tissue engineering schemes, and mesenchymal stem cells (MSCs) are emerging as an important tool in bone tissue regeneration. Although several soluble signals influencing osteogenic differentiation of MSCs in vitro are well-characterized, relatively little is known about the influence of substrate signals. This study was aimed at elucidating the effects of a bone-like mineral (BLM), which is vital in the process of bone bonding to orthopedic implant materials, on the osteogenic differentiation of human MSCs in vitro. Growth of a BLM film (carbonate apatite, Ca/ P=1.55) on poly(lactide-co-glycolide) (PLG) substrates was achieved via surface hydrolysis and subsequent incubation in a modified simulated body fluid. The BLM film demonstrated significantly increased adsorption of fibronectin, and supported enhanced proliferation of human mesenchymal stem cells (hMSCs) relative to PLG substrates. In the absence of osteogenic supplements hMSCs did not display a high expression of osteogenic markers on BLM or PLG. In the presence of osteogenic supplements hMSCs exhibited greater expression of osteogenic markers on PLG substrates than on BLM substrates, as measured by alkaline phosphatase activity and osteocalcin produ