Identification of chicken, duck, pigeon and pig.pdf

Meat Science 83 (2009) 454–459Contents lists available at ScienceDirect Meat Science journal homepage: www.elsevier .com/ locate/meatsc iIdentification of chicken, duck, pigeon and pig meat by species-specific markers of mitochondrial origin Santosh Haunshi a,*, Rantu Basumatary b, P.S. Girish c, Sunil Doley a, R.K. Bardoloi b, Ashok Kumar d aMolecular Genetics Laboratory, Poultry Science Division, ICAR Research Complex for N.E.H. Region, Umroi Road, Umiam 793103, Meghalaya, India bAnimal Production Division, ICAR Research Complex for N.E.H. Region, Umroi Road, Umiam 793103, Meghalaya, India cNational Research Centre on Meat, Chengicherla, PB No. 19, Uppal Post, Hyderabad 500039, AP, India dAnimal Health Division, ICAR Research Complex for N.E.H. Region, Umroi Road, Umiam 793103, Meghalaya, Indiaa r t i c l e i n f o Article history: Received 20 December 2008 Received in revised form 2 May 2009 Accepted 15 June 2009 Keywords: Chicken Duck Pigeon Pig PCR Mitochondria Speciation Marker0309-1740/$ - see front matter  2009 Elsevier Ltd. A doi:10.1016/j.meatsci.2009.06.026 * Corresponding author. Tel.: +91 364 2570305; fax E-mail address: santoshi575@ (S. Hauna b s t r a c t In the present study, PCR based method for meat species identification of chicken, duck, pigeon and pig was achieved by developing species-specific markers. Using mitochondrial sequences species-specific primers were designed and the sizes of them were 256 bp, 292 bp, 401 bp and 835 bp for chicken, duck, pigeon and pig, respectively. The species-specific PCR products were sequenced to confirm the specificity of the product amplified. These markers were subsequently tested for cross amplification by checking them with beef, mutton, chevon, pork, rabbit, chicken, duck, turkey and pigeon meat. DNAmarkers devel- oped in this study can help identify the species of fresh, cooked and autoclaved meat of chicken, duck and pigeon and fresh and cooked meat of pig. The process of identification is simple, econo