Box–Behnken design based statistical modeling for ultrasound-assisted.pdf

B e J a b a A R R A A K U P C B E 1 a a i o b w K C Z c W a d p b f c m ( d 0 hCarbohydrate Polymers 92 (2013) 604– 611 Contents lists available at SciVerse ScienceDirect Carbohydrate Polymers jou rn al hom epa ge: www.elsev ier .com/ locate /carbpol ox–Behnken design based statistical modeling for ultrasound-assisted xtraction of corn silk polysaccharide . Prakash Marana,?, S. Manikandanb, K. Thirugnanasambandhama, C. Vigna Nivethaa, R. Dinesha Department of Food Technology, Kongu Engineering College, Perundurai, Erode 638052, TN, India Department of Food and Process Engineering, SRM University, SRM Nagar, Kattankulathur, Chennai 603203, TN, India r t i c l e i n f o rticle history: eceived 8 August 2012 eceived in revised form 30 August 2012 ccepted 9 September 2012 vailable online 16 September 2012 a b s t r a c t In this study, ultrasound assisted extraction (UAE) conditions on the yield of polysaccharide from corn silk were studied using three factors, three level Box–Behnken response surface design. Process param- eters, which affect the efficiency of UAE such as extraction temperature (40–60 ?C), time (10–30 min) and solid–liquid ratio (1:10–1:30 g/ml) were investigated. The results showed that, the extraction con- ditions have significant effects on extraction yield of polysaccharide. The obtained experimental dataeywords: ltrasound olysaccharide orn silk ox–Behnken design xtraction were fitted to a second-order polynomial equation using multiple regression analysis with high coef- ficient of determination value (R2) of 0.994. An optimization study using Derringer’s desired function methodology was performed and the optimal conditions based on both individual and combinations of all independent variables (extraction temperature of 56 ?C, time of 17 min and solid–liquid ratio of 1:20 g/ml) were determined with maxi