Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens.pdf

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Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens.pdf

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Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens

D a X a b c d a A R R 1 A K E B F D B 1 t s l S E s a t m i s H a i a s b 0 dJournal of Biotechnology 140 (2009) 38–44 Contents lists available at ScienceDirect Journal of Biotechnology journa l homepage: www.e lsev ier .com/ locate / jb io tec ifficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens re efficient in controlling fire blight disease .H. Chena, R. Scholza, M. Borrissb, H. Jungeb, G. M?gelc,d, S. Kunzc,d, R. Borrissa,? Institut für Biologie, Humboldt Universit?t Berlin, Chausseestrasse 117, D10115 Berlin, Germany ABiTEP GmbH, Glienicker Weg 185, 12489 Berlin, Germany Bio-Protect GmbH, Lohnerhofstra?e 7, 78467 Konstanz, Germany Universitaet Konstanz, LS Phytopathologie, Universit?tsstrasse 10, D-78457 Konstanz, Germany r t i c l e i n f o rticle history: eceived 25 July 2008 eceived in revised form 9 September 2008 ccepted 21 October 2008 eywords: a b s t r a c t Representatives ofBacillus amyloliquefacienswere shown topossess biocontrol activity against fire blight, a serious disease of orchard trees caused by Erwinia amylovora. Genome analysis of B. amyloliquefaciens FZB42 identified gene clusters responsible for synthesis of several polyketide compounds with antibac- terial action. We show here that the antibacterial polyketides difficidin and to a minor extent bacillaene act efficiently against E. amylovora. Surprisingly, a mutant strain blocked in the production of difficidin (CH8 dfn) inhibited growth of E. amylovora and suppressed fire blight disease nearly in the same rangerwinia amylovora acillus amyloliquefaciens ire blight control ifficidin acilysin as the wild type. In addition, a sfpmutant (CH3sfp) unable to synthesize non-ribosomally lipopeptides and polyketides did still suppress growth of E. amylovora, suggesting that besides action of polyketides another antagonistic principle exist. Adoublemutant (RS06sfpbac) devoid inpolyketide andbacilysin synthesis was unable to suppress growth of E. amylovora indicating th