雌孕激素对大鼠前列腺增生的影响.docVIP

雌孕激素对大鼠前列腺增生的影响 张燕 韦哲 连溯 王晨光 贾倩倩 王丽莎 李杏 迟博 林颖 指导老师：潘尚领 （广西医科大学临床医学院，广西南宁市，530021） 摘要：目的 探讨不同比例雌孕激素对雄性大鼠前列腺的影响。方法 将48只成年的雄性大鼠随机分为对照组和不同比例的雌/孕激素组（10:100，10:50，10:25，10:5，10:0），每组各8只，实验组每日于相同时间按计算比例肌注雌孕激素一次。对照组于每日相同时间肌注相同量的生理盐水。肌注6周后取前列腺标本，计算各组的前列腺指数，观察各组的组织切片的形态学变化。 结果 与对照组相比较，不同比例雌/孕激素组前列腺指数明显降低，不同比例雌孕激素较正常前列腺组织萎缩明显，但实验组各组间差异无显著性。结论 联合应用雌/孕激素可致正常大鼠前列腺萎缩，为临床治疗前列腺增生提供参考。 关键词：雌激素 孕激素 前列腺 中图分类号：R697 Effect of estrogen/progesterone on rat prostate Yan Zhang, Zhe Wei, Su Lian, Chenguang Wang, Qianqian Jia, Lisha Wang, Xing Li, Bo Chi, Ying Lin, Shangling Pan (School of Clinical Medicine, Guangxi Medical Uniersity, Nanning 530021, Guangxi, China) Abstract Objective To investigate the effect of different proportion of estrogen/progesterone (E2/P) on rat prostate. Methods Forty eight male rats were randomly divided into five experimental groups and one control group. Each group includes 8 rats. The experimental groups were injected with estrogen/progesterone which proportion was 10:100，10:50，10:25，10:5，10:0 respectively, the same volume of normal saline was given to the controls. Prostate index (PI) and histology were observed after 6 weeks’ of exposure to female hormone. Results Compared to the control group, the PIs of experimental groups were reduced distinctly. The prostate tissue of experimental groups was more atrophic than the controls. However, there was no variance among the experimental groups. Conclusion Combining use of estrogen/progesterone can lead to prostate atrophic for normal rats, suggesting an alternative solution in treating prostate hypertrophy. Key words: Estrogen; Progesterone; Prostate 1. 材料与方法 1.1 动物与药品 Wistar大白鼠48只，雄性，体重140-170g，由广西医科大学动物实验中心提供。苯甲酸雌二醇注射液（Estradiol Benzoate Injection,上海通用药业股份有限公司，国药准字；黄体酮注射液（Progesterone Injection 浙江仙琚制药股份有限公司。药准字。0.9%NaCl,由广西医科大学第一附属医院药剂室提供。 1.2 方法 1.21 饲养及用药 将48只大鼠随机分为正常对照组和不同比例的E2/P组（10:100，10:50，10:25，10:5，10:0）共计6组，每组8只。各组均自由取食和自由饮水。于每日固定时间肌注药物一次，各实验组按计算计量于动物固定后将药物注射于动物后肢大腿外侧肌肉内，左右后肢每