聚焦超声扫描对免气管黏膜的影响.pdfVIP

中黯霉鼻暗礁拱旗外科12009年4月，第16巷，第4期 青在 弘 ? it 11 喻 叩 q q 磁 聚焦超声扫描对兔气管黠膜的影响 张酷辑te2，韩憾盹2.3，王琪2，张罗2. 3 (1 首都应科大学附踹北京向忆医院 12000摄7年制帧士研究焦.2 耳鼻H自喉头2窗外料，耳鼻咽喉头贺科学教商部重点实验室 (首都阻科大学) ，才t京 100730; 3 北京市耳鼻咽喉科研究所，北京 100005) i摘要} 目前 黯嚷囊焦超声扫描对兔气管黠攘的影 响。方法 对陶体兔气管进行聚焦制声相捕，即剖行组 织脚理学观棋黠膜形态学政变，罔时另部分兔气管行组 棋块法， 5在期离速数字化摇旗成穰技术，黯赣好毛摆动 频郁，比较使用黯焦起商扫描盾的兔气管上庶奸毛摆动 频率与正常兔气管纤毛攒动频率是否有茬异。锚果 聚 集超声扫描爵的兔气管鞍旗奸毛柱状上皮结特汪常，黠 腆下摆出现水肿，散在凝固坏死，血管内皮细胞变性， 腺体细胞部分坏死。聚焦趣商扫描瞒的纯气瞥上应纤毛 摆动攘攘主否正常兔气蟹纤电摆动频率先提著性若是异。结 论 聚焦起声拍描对辈古自费辍展功能有维萨作用，而对黯 膜下底细织有做坏作用。 {关键晦; 越南疗法;睁眼道黯膜;黠膜好毛清除;离 强戴焦 Effects of focused ultrasound wave on mucosa of rabbit trachea E仙NG剧lengl.2， HAN C加m的2， 3， WANGC矿，刷刷GμKf 3 (1 2000 Post-Graduate Student, 2 Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital , Capital Medìcal Univer岱ity ， Key Laboratory of Otolaryngology 州ead and Neck Surgery (Capítal M~dical University) , Ministery of Education，日eijing ， 100730, China; 3 日eijing Institute of Otolaryngology, Beijing, 100005, Chìna) Corresponding author: ZHANG Luo (Email: luozhang @trhos.∞m) [A日STRACT] OBJECTIVE To study the effect of focused ultrasound wave on lower respiratory and 篡金项~: ~翻滚囊点篡础研究发展计划~ (973 黯剿， 2007CB516706) ，因家自然科学基金 和教育部新世 纪优秀人才计划 (NCET-06-0185) 联合资助 第一作将简介及通讯:张院盘，骋，北京人，医学硕士，::1:主要研究方 向为草草科学. 通讯作者:张罗侄maíl: luozhang@trhos.∞m) trachea mucosa of rabbit. METHODS Focused ultrasound 5can wa8 performed on rabbit trachea in vitro唰 Pathological changes of tracheal mUC05a were investigated. Part of trachea mucosa was seeded on collagen翩coated cover slips for primary culture. Phase. contrast microscopy was used to detect the cilia activity.他 τhe phase心ontrast im吨es were captured with a high- speed digital camera whích was a progressive 8can charged翩coupled device (CCD) that provided images at 240 frames per second (fps) and then to detect the gra沪intensity variation in the region of ìnterest (ROI) . RE5ULTS Ught microsωpic examination showed that the epithelia of tracheal mucosa was intact and the