Culturable Psychrotrophic Bacterial Communities in Raw Milk and Their Proteolytic and Lipolytic.pdf

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Culturable Psychrotrophic Bacterial Communities in Raw Milk and Their Proteolytic and Lipolytic.pdf

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Culturable Psychrotrophic Bacterial Communities in Raw Milk and Their Proteolytic and Lipolytic

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Nov. 2007, p. 7162–7168 Vol. 73, No. 22 0099-2240/07/$08.000 doi:10.1128/AEM.00866-07 Copyright ? 2007, American Society for Microbiology. All Rights Reserved. Culturable Psychrotrophic Bacterial Communities in Raw Milk and Their Proteolytic and Lipolytic Traits? Elionora Hantsis-Zacharov1 and Malka Halpern1,2* Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mount Carmel, Haifa 31905, Israel,1 and Department of Biology, Faculty of Science and Science Education, University of Haifa, Oranim, Tivon 36006, Israel2 Received 17 April 2007/Accepted 10 August 2007 During cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. The diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. About 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in raw milk. The psychrotrophic isolates were identified and classified in seven classes. Three classes were predominant, with high species richness (18 to 21 species per class) in different seasons of the year: Gammaproteobacteria in spring and winter, Bacilli in summer, and Actinobacteria in autumn. The four minor classes were Alphaproteobacteria, Betaproteobacteria, Flavobacteria, and Sphingobacteria. The dominant classes were found in all four dairies, although every dairy had its own unique “bacterial profile.” Most but not all bacterial isolates had either lipolytic or both lipolytic and proteolytic activities. Only a few isolates showed proteolytic activity alone. The dominant genera, Pseudomonas and Acinetobacter (Gammaproteobacteria), showed mainly lipolytic activity, Microbacterium (Actinobacteria) was highl