High-resolution in situ hybridization to whole-mount zebrafish embryos.pdfVIP

High-resolution in situ hybridization to whole-mount zebrafish embryos Christine Thisse Bernard Thisse Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA. Correspondence should be addressed to C.T. (christhisse@) or B.T. (bernardthisse@). Published online 20 December 2007; doi:10.1038/nprot.2007.514 The in situ hybridization (ISH) technique allows the sites of expression of particular genes to be detected. This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-moun