外源性干细胞因子对糖尿病胃轻瘫模型大鼠胃电及胃阻抗的影响.docVIP

外源性干细胞因子对糖尿病胃轻瘫模型大鼠胃电及胃阻抗的影响 高金亮，赵 楠，宋 展(南阳市中心医院，河南省南阳市 473009) 引用本文：高金亮，赵楠，宋展. 外源性干细胞因子对糖尿病胃轻瘫模型大鼠胃电及胃阻抗的影响[J].中国组织工程研究，2017，21():128-132. DOI: 10.3969/j.issn.2095-4344.2017.01.023 ORCID: 0000-0001-6805-1347(高金亮) 文章快速阅读： 文题释义： Cajal间质细胞8周后检测胃内色素残留率，透射电镜观察胃窦组织微结构。造模成功后，观察组腹腔注射干细胞因子0.4 μg/(kg?d)，对照组腹腔注射治疗相同剂量的磷酸盐缓冲液，均连续给予15 d，治疗结束后进行阻抗式胃动力系统检测。 结果与结论：①观察组胃内色素残留率显著高于对照组(P 0.05)；②观察组大鼠胃窦Cajal间质细胞间缝隙连接显著减少，呈松散连接状，基膜结构缺乏连续性，细胞内的细胞器数量显著减少，胞质溶解，出现大量的空泡；③观察组大鼠治疗后的胃阻抗以及胃电情况均得到显著改善，胃电 2频段功率百分比显著下降，2-4频段功率百分比显著上升(P 0.05)；胃阻抗 2频段功率(胃动过缓)百分比显著下降，2-4频段功率百分比显著上升(P 0.05)。治疗后不同频段功率与对照组之间差异无显著性意义(P0.05)；④结果表明，糖尿病胃轻瘫大鼠胃排空会出现明显的延迟现象，Cajal间质细胞变性可能是胃轻瘫出现的原因。外源性干细胞因子可以有效改善大鼠的胃电和胃阻抗水平。 Effects of exogenous stem cell factors on the gastric electrical activity and impedance in a mouse model of diabetic gastroparesis Gao Jin-liang, Zhao Nan, Song Zhan (Nanyang Central Hospital, Nanyang 473009, Henan Province, China) Abstract BACKGROUND: Lesions in Cajal interstitial cells can lead to diabetic gastroparesis, in which, insulin and stem cell factor are likely to act as upstream regulatory factors. OBJECTIVE: To investigate the effect of exogenous stem cell factors on the gastric electrical activity and gastric impedance in a mouse model of diabetic gastroparesis. METHODS: Forty Wistar rats were randomly divided into two groups, 20 rats in each group. The diabetic gastroparesis model was prepared in observation group by a single use of 60 mg/kg streptozotocin via intraperitoneal injection and high-sugar/high-fat diet. Rats in control group were given the equal volume of normal saline and normal diet. Eight weeks later, residual rate of gastric pigment was detected, and gastric antrum microstructures were observed under transmission electron microscope. If the animal model was successfully prepared, rats were intraperitoneally given 0.4 μg/kg/d stem cell factor in the observation group and same volume of PBS in the control group, for consecutive 15 days. After