新斯的明经穴位注射对大鼠关节疼痛的镇痛作用及机制研究.pdf

Traditional Chinese Medicine 中医学, 2016, 5(1), 12-18 Published Online February 2016 in Hans. /journal/tcm /10.12677/tcm.2016.51003 Analgesic Efficacy and Mechanism of Acupoint Injection of Neostigmine in a Rat Model of Joint Pain Miao Zhang*, Xuran Zhang No. 3 Surgical Ward, Department of Orthopaedics, Fuxin Central Hospital, Fuxin Liaoning Received: Jan. 27th, 2016; accepted: Feb. 16th, 2016; published: Feb. 19th, 2016 Copyright ? 2016 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Objective: Neostigmine could provide peripheral analgesic effects via intra-articular injection, but with obvious technical difficulty and potential complications. The purpose of this study is to inves- tigate the analgesic effect and mechanism of acupoint injection of neostigmine in a rat model of joint pain. Methods: Animal models of ankle joint pain were prepared via intra-articular injection of monosodium iodoacetate in male Wistar rats. The experimental group received acupoint injec- tion of neostigmine while the control group received acupoint injection of normal saline. The numbers of hind paw licking and radiation pain threshold were recorded, and serum IL-1β and TNF-α were measured using ELISA. Results: Compared with the control group, the animals in the experimental group presented less hind paw licking, higher radiation threshold and lower levels of serum IL-1β and TNF-α (p 0.01). Conclusion: Acupoint injection of neostigmine elevated the radiation pain threshold and lowered the level of proinflammatory pain medi