分子信标技术李舒艳200425038.ppt

分子信标技术;分子信标简介 分子信标的结构 分子信标的工作原理 分子信标的应用 前景与展望;分子信标简介;分子信标的结构;首例分子信标结构示意图;分子信标工作原理;影响分子信标的因素; 温度对分子信标的影响;波长转移分子信标;;表面固定分子信标;量子点分子信标;;双链DNA检测;Human osteosarcoma cell nucleus (U2OS cell line) vitally injected with a Cy3-labelled probe specific for chromosomal telomeric repeat sequenes. Note that some spots are relatively large suggesting association or clustering of multiple telomeres.;　　　Whitcombe设计的一种将ＰＣＲ引物与分子信标相结合的蝎状引物荧光探针。在该探针中,ＰＣＲ引物与分子信标之间有一间隔臂,使ＰＣＲ反应不能往分子信标方向延伸。在ＰＣＲ过程中,非特异扩增产物不会产生荧光信号,而当特异性扩增产物生成时,分子信标将立即与其进行分子内杂交,同时发出荧光信号。;;;Working mechanism of the fluorophore–quencher-labeled MAB. The MAB exists at equilibrium between a nonstructured random coil and a compact intramolecular quadruplex. Addition of thrombin (gray ellipse) shifts the equilibriumin favor of the quadruplex structure, which draws the fluorophore (F) and quencher (Q) closer, leading to fluorescence quenching. The working mechanism of the donor–acceptor-labeled MAB is similar to the quench-type MAB. The arrows of the backbone of the oligonucleotidesindicate the 5–3 polarity of DNA.;Chaoyong James Yang et al, Chaoyong James Yang, Mauricio Pinto, Kirk Schanze,and Weihong Tan*.Angew. Chem. Int. Ed. 2005, 44, 2572 –2576;前景与展望;Thank you!