系统性红斑狼疮患者外周血淋巴细胞CD43抗原的研究x.docxVIP

网址： HYPERLINK  第  PAGE 4 页，共  NUMPAGES 4 页 系统性红斑狼疮患者外周血淋巴细胞CD43抗原的研究 系统性红斑狼疮患者外周血淋巴细胞CD43抗原的研究 中华皮肤科杂志 1998年第1期第31卷 论著 作者：梁再赋 张士发 许静 冯永山 王壮志 顾绍裘 单位：110001 沈阳，中国医科大学第一临床学院皮肤科(梁再赋　许静　冯永山　王壮志　顾绍裘)；沈阳军区总医院皮肤科(张士发) 关键词：红斑狼疮；系统性；流式细胞计数 【摘要】 目的 探讨SLE免疫功能异常的原因。方法 采用免疫荧光双标记-流式细胞仪检测了16例活动期SLE患者外周血淋巴细胞的CD43抗原。结果 ①活动期SLE患者外周血CD43抗原阳性淋巴细胞为63.14±16.47，低于正常人对照组86.62±12.86，有非常显著性差异(P＜0.01)；②CD43抗原阳性淋巴细胞数与SLE疾病活动度呈显著负相关，r=-0.702，P＜0.01；③活动期SLE患者外周血CD19+-CD43+淋巴细胞为2.55±1.38，高于对照组1.32±0.92，有非常显著性差异(P＜0.01)。结论CD19+-CD43+淋巴细胞的增多可能与SLE大量产生抗DNA自身抗体有关。 CD43Preliminary Study of Expression of CD43 Antigen on lymphocytes of Peripheral Bloodin Patients with SLE Liang Zaifu, Zang Shifa, Xu Jing, et al. department of Dermatology,No.1 Hospital of China Medical University,Shenyang 110001 【Abstract】 Objective To investigate the causes of immunological disorders in SLE.Methods CD43 antigen on lymphocytes of peripheral blood in 16 cases of active SLE was detected by immunoflourescent double marker-flowcytometry.Results The results showed that, (1)the number of CD43 antigen positive lymphocytes in peripheral blood of active SLE patients was 63.14 ± 16.47, which was significantly lower than that of the control group(86.62 ± 12.86, P＜ 0.01). (2) The number of CD43 antigen positive cells was negatively correlated to the disease activity of SLE (r=-0.702,P＜0.01). (3) The number of CD19+-CD43+ lymphocytes in peripheral blood in active SLE was 2.55±1.38 which was significantly higher than that of the control group (1.32±0.92, P＜0.01). Conclusion The increase of CD19＋- cD43＋ lymphocytes may be correlated with the production of anti-DNA autoantibody in SLE. 【Key words】 Lupus erythematosus,systemic Flowcytometry CD43 SLE发病机理尚未完全阐明，一般认为SLE患者存在免疫功能紊乱而表现出调节性T细胞功能异常及B细胞多克隆活化。CD43抗原是与淋巴细胞活化相关的大分子糖蛋白，我们拟通过观察CD43分子在SLE患者中的变化来探讨SLE免疫学发生机理。 材料和方法 (一)研究对象：SLE患者16例为本院住院患者，按美国风湿病协会1982年标准确诊，平均年龄29.88岁(15～46岁)；平均病程28个月；均为活动期患者，其疾病活动