采用连续分级提取法巢湖沉积物中不同结合态氮的赋存特征5.13讲述.doc

采用连续分级提取法研究春季巢湖沉积物中不同结合态氮的赋存特征收稿日期: 基金项目: 国家自然科学基金(200507017) 作者简介: 钟立香(1984-)，山东潍坊人，在读硕士，主要从事湖泊水环境方面的研究。E-mail：newlycoco@163.com *责任作者：姜霞, E-mail：jiangxia@ 钟立香1，王书航1，2，姜霞1,*，金相灿1 (1.中国环境科学研究院, 北京 100012; 2.合肥工业大学资源与环境学院, 安徽 合肥 230009) 摘 要：为研究沉积物中不同形态氮的释放能力及其生物可利用性大小,以春季巢湖表层沉积物为例,采用连续分级提取法将氮分为4种形态：游离态氮(FN)、可交换态氮(EN)、酸解态氮(HN)及残渣态氮(RN)，并研究了其赋存特征.结果表明, 沉积物中总凯氏氮含量(TKN)在1 004 mg/kg～2 285 mg/kg之间,各形态氮含量大小为HN﹥EN﹥RN﹥FN,占总提取态氮比例分别为: 78.32%、11.50%、9.76%、0.42%。酸解氨基酸态氮是可矿化态氮最有效贡献者,多元逐步回归方法得到“最优”方程为：y=0.696 AAN -108.918.连续提取法测得总氮值(TSEN)比用凯氏半微量法测得总氮值(TKN)偏小,但在误差允许的范围内, TSEN可替代半微量凯氏法测得的总氮.连续分级提取法研究沉积物中氮形态为湖泊水环境生态安全评估提供基础依据. 关键词：连续提取；氮形态；沉积物；巢湖 中图分类号： Study on Speciation Characteristics of Different Combined Nitrogen in the Sediments of Chaohu Lake by Sequential Extraction Methods ZHONG Li-xiang1, WANG Shu-hang1,2, JIANG Xia1, JIN Xiang-can1 (1: Chinese Research Academy of Environmental Sciences, Beijing, 100012, PR China; 2: Hefei University of Technology, School of Resource and Environment, Hefei, 230009, PR China) Abstract：The release capability and bioavailability of different nitrogen speciation in the surface sediment of Chaohu Lake were investigated. The nitrogen speciation were divided as the free nitrogen (FN), the exchangeable nitrogen (EN), the acid hydrolysable nitrogen (HN) and the residual nitrogen (RN) by sequential extraction methods. And their concentrations in the surface sediment were also analyzed. The results showed that the TKN concentrations of surface sediments ranged from 1 004 mg/kg～2 285 mg/kg, that the concentrations of nitrogen speciation followed the order of HN﹥EN﹥RN﹥FN, their proportion to the total extractable N were 78.32%, 11.50%, 9.76% and 0.42% respectively. AAN is the most efficient contributor to the mineral N with the best regression equation of y=0.696AAN-108.918 by multiple stepwise regression method. The total sediment extractable nitrogen (TSEN) was a little smaller than the total Kjeldhal nitrogen (TKN). However TSEN could take plac