分枝杆菌的实验室诊断幻灯片1.pptVIP

Laboratory Methods for the Diagnosis of Mycobacterium分枝杆菌的实验室诊断;实验室诊断方法;课程目标 (1);课程目标 (2);General Characteristics;结核菌的培养;检体采集;分枝杆菌检体收集容器;加等量的 6% NaOH-NALC-Sodium citrate化痰液 强力 Vortex 约 15 - 20 秒 挑取约 5 ml 痰液至 50 ml 离心试管 室温静置 20 分钟 加无菌 Phosphate Buffer 至约 45 ml, 盖紧盖子 离心 3,800 rpm 约 15 分钟( 4℃) 倒掉上清液, 留下 2 ml 接种: 取 0.5 ml 检体至 MGIT tube 0.1ml检体至LJ 培养基 同时做一片抹片染 Acid Fast Stain;Date;接种的培养基;MGIT Technology;Inoculation and Incubation;Incubation;Solid Macroscope Smooth、coarse Pigment Microscope Needle;MGIT Broth Medium;Broth Morphology;Solid;Rough;Acid-Fast Stain Method;厚缩抹片制作;Kinyoun acid fast stain (cold);Interpretation (Ziehl-Neelsen/Kinyoun);Method of Examination 左右3次来回，上下9次来回 ;结核菌室鉴定试验 工作流程图 ;BDProbeTecTMET;BD ProbeTec 鉴定;BDProbeTecTMET鉴定;Mycobacterial antigen;MPB 64 Protein features;Immunochromatographic assay (ICA slide test );MPB64;TiBilia test of principle;The positive band developed within 5 to 10 min Intensity of the color was maximum at 15 min;Simple and Rapid Identification of the MTBC by ICA Using Anti-MPB64 Monoclonal Antibodies;;NTM的鉴定方法;＜7 天;2hrs;3周;Results with tests;PCR restriction-enzyme analysis (PRA) method;Agarose Electrophoresis: 4% agarose, 50V;Procedure for PRA;PCR-Mycobacteria (439bp);Lane;鉴定;Antimicrobial Susceptibility (1) BACTEC MGIT 960 SIRE;Antimicrobial Susceptibility (2);Mechanisms of Resistance;Scan the AST set into the instrument Remove the set when completed Print the report with AST interpretations of either Susceptible or Resistant ;;TB 室考题;答案:1.C 2.A 3.B 4.A5.ABCD 6.A 7.A 8.B 9.A 10.C