2012综述：合成生物学在生产生物燃料方面的应用.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2010, Article ID 761042, 18 pages doi:10.1155/2010/761042 Review Article Metabolic Engineering for Production of Biorenewable Fuels and Chemicals: Contributions of Synthetic Biology Laura R. Jarboe,1 Xueli Zhang,2 Xuan Wang,2 Jonathan C. Moore,2 K. T. Shanmugam,2 and Lonnie O. Ingram2 1 Department of Chemical and Biological Engineering, Iowa State University, 3051 Sweeney Hall, Ames, IA 50011, USA 2 Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, FL 32611, USA Correspondence should be addressed to Lonnie O. Ingram, ingram@u?.edu Received 14 August 2009; Revised 18 December 2009; Accepted 13 January 2010 Academic Editor: Patrick Cirino Copyright ? 2010 Laura R. Jarboe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Production of fuels and chemicals through microbial fermentation of plant material is a desirable alternative to petrochemical- based production. Fermentative production of biorenewable fuels and chemicals requires the engineering of biocatalysts that can quickly and e?ciently convert sugars to target products at a cost that is competitive with existing petrochemical-based processes. It is also important that biocatalysts be robust to extreme fermentation conditions, biomass-derived inhibitors, and their target products. Traditional metabolic engineering has made great advances in this area, but synthetic biology has contributed and will continue to contribute to this ?eld, particularly with next-generation biofuels. This work reviews the use of metabolic engineering and synthetic biology in biocatalyst