蛇胆川贝液的质量研究.pdfVIP

西华大学硕士学位论文 摘 要 本实验对八个不同厂家所生产的蛇胆川贝液进行薄层色谱和高效液相色谱分析，通 过检测其主要成分贝母的含量来评价其质量。在硅胶 G 薄层色谱板上点上蛇胆川贝液进 行预处理后的试液和处理后的对照品贝母，再用展开剂展开，喷上显色剂使他们斑点清 晰。将蛇胆川贝液进行衍生化处理，以贝母素甲为标准品进行高效液相色谱分析，通过 检测贝母素甲的含量来计算贝母的含量。结果发现，不同厂家的产品其共有峰较多，说 明其成分差别不大。但是贝母的用料和用量并不一致，应该建立统一的标准。用上述两 种试验方法做对比，可以为鉴定中药质量提供一定的参考依据。 关键词：蛇胆川贝液；贝母；薄层色谱；高效液相色谱；指纹图谱 I 蛇胆川贝液的质量研究 Abstract Shedan Chuanbei liquid the production of eight different manufacturers by TLC and HPLC analysis, to evaluate its quality through detecting the content of the main component of fritillaria. Shedan Chuanbei liquid pretreatment was made for, and Fritillaria processing as control article, respectively on the silica gel G thin-layer chromatography plate, an expansion of expansion agent, spray reagent makes it clear spots. The Shedan Chuanbei liquid sample derivatization, with peimine as standard for high performance liquid chromatography, by detecting the content of peimine content to calculate the fritillaria. Results showed that, different manufacturers of the products of the common peaks is more, the component difference. But the material and dosage of Fritillaria were not consistent, should establish a unified standard. Comparison of two methods for the identification of the quality of Chinese medicine to provide certain reference . Key Words：Liquid Snake Chuanbei; Fritillaria; TLC; HPLC; Fingerprints II 西华大学硕士学位论文 目 录 摘 要 ............................................................................................................................. I Abstract ....................................................................................................