成神经分化的骨髓间充质干细胞向C6细胞条件培养基和SDF1α的趋化性迁移研究的论文.docVIP

　　成神经分化的骨髓间充质干细胞向C6细胞条件培养基和SDF1α的趋化性迁移研究的论文 【摘要】 目的: 探讨成神经分化的骨髓间充质干细胞(bmscs)向c6细胞条件培养基和sdf1α的趋化性迁移。方法: 采用碱性成纤维生长因子(bfgf)、 丁羟基茴香醚(bha)、 二甲基亚砜(dmso)联合诱导剂对bmscs进行诱导分化, 通过免疫荧光染色检测诱导的细胞表达神经前体细胞标志物nestin、 βiiitubulin和nse的情况。运用dunn chamber研究成神经分化的bmscs向胶质瘤和sdf1α的迁移。结果: bmscs能诱导分化成神经元样细胞, 而对照组的bmscs形态无变化。dunn chamber分析显示, c6细胞条件培养基组和sdf1α组诱导细胞的迁移速率和迁移效率明显高于对照组, 表明c6细胞条件培养基和sdf1α对bmscs具有趋化作用, 单个细胞迁移轨迹实验也证实了这一结果。此外, 分化不同状态的bmscs向c6细胞条件培养基和sdf1α的趋化程度也不同。结论: bmscs的???向迁移与分化状态密切相关。 【关键词】 骨髓间充质干细胞; 分化; 迁移; 趋化性 tropism of bone marroesenchymal stem cells in neurogenic differentiation for c6 conditioned medium and sdf1α 　　bao puhua, in, su jun, liu mengyu, zhang huanxiang* 　　department of cell biology, medical college of soocho cell research, suzhou 215123, china 　　[abstract] aim: to investigate the tropism of bone marroesenchymal stem cells (bmscs) in neurogenic differentiation for glioma and sdf1α. methods: bha, bfgf, and dmso scs, and the expression of neural markers, nestin, βiiitubulin and nse munocytochemistry. ber, the migration of bmscs in neurogenic differentiation toa and sdf1α. results: bmscs could be induced to differentiate into neuronlike cells, ained the morphology of bmscs. dunn chamber analysis revealed that both the migration speed and efficiency of cells induced by c6 conditioned medium and sdf1α onstrating the chemotactic effect of both c6 conditioned medium and sdf1α on bmscs. these results ed by examination of migration tracks of individual cells. moreover, bmscs at different states of differentiation sho for c6 conditioned medium and sdf1α. conclusion: the directed migration of bmscs is closely related to the differentiation states of these cells. 　　[keyscs; differentiation; migration; tropism 恶性胶质瘤是目前最为盛行的脑瘤, 干细胞移植极有希望治疗胶质瘤。.因骨髓间充质干细胞(bone marroesenchymal stem cells, bmscs)体内体外均显示很强的胶质瘤趋化性[1], 所以应用bmscs治疗胶质瘤即是很好的细胞治疗手段。然而处于不同分化状态的bmscs, 尤其是成神经分化过程中的bmscs能否改变其向胶质瘤趋化性迁移的能力, 还不得而知。众多研究表明, c6细胞分泌的多种细胞因子影响bmscs的定向迁移, 如sdf1α。鉴于此, 本实验利用化学诱导剂联合