-1 Rat heme oxygenase expression of ventilator-associated lung injury SOD MDA of.docVIP

 PAGE \* MERGEFORMAT 16 -1 Rat heme oxygenase expression of ventilator-associated lung injury SOD MDA of [Abstract] Objective in rats with ventilator-associated lung injury (VILI) as a model, with hemin (hemin) induced heme oxygenase -1 (HO-1) expression was observed when superoxide dismutase VILI enzyme (SOD) and malondialdehyde (MDA) and HO-1 activity of SOD and MDA explore the effect of the process in VILI protective effect against oxidative stress and its mechanism. Methods 32 male SD rats were randomly divided into 4 group (each group n = 8): control group, only tracheotomy, spontaneous respiration; model group underwent tracheotomy with mechanical ventilation 4 h; inductor group on the model prepared by intraperitoneal injection 24 h before hemin 40 mol / kg; inhibitor group in the model by intraperitoneal injection 24 h before the preparation of zinc protoporphyrin (ZnPP) 10 mol / kg. mechanically ventilated rats were killed after 4 h to collect lung tissue and bronchoalveolar lavage fluid (BALF) samples were determined BALF total protein content, lung wet / dry weight ratio (W / D), lung tissue LDH, SOD activity and MDA content in lung tissue HO-1 protein expression, light microscope observation of lung tissue pathology. The results with the control group, model group, severe pathological damage lung tissue, lung W / D, BALF total protein, LDH activity was significantly increased, VILI model reproduced successfully. Compared with model group, lung tissue inducer increased expression of HO-1 , significantly reduced lung tissue damage, SOD activity was significantly increased MDA content was significantly decreased, with ZnPP inhibited HO-1 expression, this protective effect disappeared. Conclusions hemin induced HO-1 expression can increase SOD activity, decreased MDA content and reduce oxidative stress of lung injury, reduced the extent of VILI. [Keywords:] ventilator-associated lung injury; Heme oxygenase -1; superoxide dismutase; malondialdehyd