-GT enzymatic properties of peptide.docVIP

 PAGE \* MERGEFORMAT 12 -GT enzymatic properties of peptide Of: Jiangmin Li, Meng Peipei, Gao Peng, Hu Liyong [Abstract] Objective licheniformis bacterial cells -glutamyl GGT some of the basic enzymatic properties. Methods Broken by lysozyme cell wall, obtained by ammonium sulfate precipitation of crude enzyme, after dialysis, the use of UV spectrophotometry Determination of enzyme activity. Results enzyme in 25 ~ 35 temperature range are well tolerated, under alkaline conditions to maintain a certain activity, the optimal reaction temperature is 45 , optimum pH, to 8.0, Ca2 +, Mg2 + and other metal ions in appropriate concentrations could promote the enzyme activity. Conclusions The use of the enzyme for the transformation of production provided the basis of theanine. [Keywords:] -glutamyl GGT; characterization; Theanine Abstract: Objective To understand some basic properties of-glutamyltranspeptidase from Bacillus licheniformis.Methods Lysozyme were used to disrupt the cell wall and ammonium sulfate were used to separate precipitation and dialysis, at last the crude enzyme were obtained. UV spectrophotometry were used to measured the enzyme activity. Results This enzyme was well tolerant to temperature in the range of 25 ~ 35 , but in alkaline conditions, it maintained a certain activity, the optimum reaction temperature was 45 and optimum reaction pH 8.0, generally the appropriate concentration of metal ions, such as Ca2 +, Mg2 +, had a positive effect on the activity. Conclusion This experiment could be used in production of theanine with -glutamyltranspeptidase. Keywords:: -glutamyltranspeptidase; enzymological property; L-theanine -glutamyl GGT (-glutamyltranspeptidase, -GTP, EC ), also known as -glutamyl transferase, widespread in vivo, is glutathione (GSH) metabolism in critical one enzyme. The enzyme is also involved in transmembrane transport of amino acids may be specific catalytic -glutamyl acyl transfer reaction [1]. people -GTP