1,1 - diphenyl-picryl hydrazine radical -2-2 Determination of transfer of spleen Hu Xin Fang and free radical scavenging capacity of different components.docVIP
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1,1 - diphenyl-picryl hydrazine radical -2-2 Determination of transfer of spleen Hu Xin Fang and free radical scavenging capacity of different components
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1,1 - diphenyl-picryl hydrazine radical -2-2 Determination of transfer of spleen Hu Xin Fang and free radical scavenging capacity of different components
[Abstract] Objective To study the transfer of spleen Hu Xin-side free radical scavenging activity of their constituents. Methods were used ethanol or water extract heat transfer spleen Hu Xin Fang ingredients, and with petroleum ether, chloroform, ethyl acetate and butanol in turn extraction, the use of 1,1 - diphenyl-picryl hydrazine -2-2 free France (DPPH method) free radical scavenging activity in experiments with both synthetic anti-oxidants vitamin C as control. The results of each component there is a certain role in scavenging free radicals, and the transfer of spleen Hu Xin Fang water extracts of the ethyl acetate extract fraction of the highest free radical scavenging activity. Conclusion tune spleen Hu Xin Fang is the main component of the polyphenolic antioxidant active ingredients, it is worth further study.
[Keywords:] transferred spleen Hu Xin Fang free radical scavenging activity of 1,1 - diphenyl-picryl hydrazine -2-2
Abstract: ObjectiveTo investigate the free radical scavenging activities and principals of regulating spleen and nourishing heart formula.MethodsThe water-extracted and regulating spleen and nourishing heart formula were separated by the order of ligarine, chloroform, ethyl acetate, and n-butano1. The antioxidant activity of regulating spleen and nourishing heart formula and its fractions were investigated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. ResultsAmong them, the ethyl acetate fraction of water extract had the most potent antioxidative effect.ConclusionThe polyphenols from regulating spleen and nourishing heart formula are main antioxidative compounds and worthy of further research.
Keywords:: Regulating spleen and nourishing heart formula; Free radical scavenging activity; DPPH
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