119 cases of chronic myeloid leukemia CML karyotype analysis.docVIP

 PAGE \* MERGEFORMAT 13 119 cases of chronic myeloid leukemia CML karyotype analysis Author: Zhu Yu, Jian-Yong Li, Jin-Lan Pan, Wu Wei, Hai-Rong Qiu, Wuya Fang, Yang Hui, Jian-Fu Zhang, Li-Juan Chen, Yong-Quan Xue 【Abstract】 In order to explore the chronic myeloid leukemia blastic phase (CML-BC) and significance of karyotype variation and application R-banding technique 119 cases of CML-BC patients with karyotype analysis of data, and randomly selected 28 cases in which the Fluorescence in situ hybridization (FISH) detection of derivative chromosome 9 [der (9)] is missing. The results showed: 124 cases of Ph-negative karyotype examination in 11 cases (8.9%), Ph-positive 113 cases (91.1%), of which 104 cases of typical translocation (83.9%), simple variant translocations in 4 cases (3.2%), complex variant translocations in 5 cases (4.0%); Ph-negative CML-BC in 72.6% of patients with additional chromosomal abnormalities, the main types are: i (17q), 14, Ph-positive 72.3% of patients with additional chromosomal abnormalities, the main types are: Ph, 8, i (17q); Ph-negative CML-BC CML time of 2-66 months, an average of 29.0 months, Ph-positive CML time of 1-127 months, an average of 34.2 months, no significant difference between the two groups (P gt; 0.05); FISH detected 5 cases (5 / 28, 17.9%) der (9) deletion. Conclusion: Chronic myeloid leukemia CML more common additional chromosomal abnormalities, FISH technology can effectively detect the der (9) deletion. Keywords: chronic myeloid leukemia; chronic myeloid leukemia blastic phase; karyotype analysis; derivative chromosome 9 deletion Karyotype Analysis in 119 Patients with Chronic Myeloid Leuke-mia at Blast Crisis Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People Hospital, Nanjing 210029, China; 1The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China AbstractT