15 1 lipoxygenase in the expression of AGS gastric cancer cells.docVIP

 PAGE \* MERGEFORMAT 17 15 1 lipoxygenase in the expression of AGS gastric cancer cells Of: Linfern, Esmond, Fong-Lin Chen, Chih Chen new Wang Chung [Abstract] Objective To study the 15-lipoxygenase 1 (15 LOX 1) gene in human gastric AGS cells and its expression in the proliferation and apoptosis of AGS. Methods Liposome-mediated transient transfection of 15 LOX 1 gene in AGS in culture, reverse transcriptase PCR, Western blotting and 15 LOX 1 mRNA and protein expression, tetrazolium salt (MTT, flow cytometry, AnnexinV / PI staining and in situ end labeling (TUNEL transfected AGS compared proliferation and apoptosis. Results AGS gastric cancer cells after transfection of the expression of 15 LOX 1 mRNA and protein. with non-transfected group and empty vector transfected group compared with recombinant plasmid transfected AGS cells significantly inhibited the growth and cell cycle arrest in G1, apoptosis was significantly higher than non-transfected group and empty vector transfected group. Conclusion 15 LOX 1 gene can inhibit the proliferation of AGS gastric cancer cells and promote their apoptosis. [Keywords:] arachidonic acid salt of 15 lipoxygenase, gastric cancer, tumor cells, cultured, cell proliferation, apoptosis 15 Lipoxygenase 1 (15 lipoxygenase 1,15 LOX 1) is a lipoxygenase isozymes, is the body of arachidonic acid catalyzed formation of reactive molecules and cell signaling and metabolism key enzyme that regulates Malignant epithelial tumors in particular, survival. To understand the 15 LOX 1 gene and gastric cancer, the present study, cell lines in vitro gene transfer method to study the 15 LOX 1 gene transfection on cancer cell proliferation and apoptosis to of the gene and the relationship between the development of gastric cancer and gene therapy as a possible candidate gene. 1 Materials and methods 15 LOX 1 1.1 Reagents Recombinant eukaryotic expression vector pcDNA3.1 (+) / 15 LOX 1 from the NIH’s Dr. Eling kindly, pcDNA3.1 (+