1502 cases of respiratory tract infection analysis of mycoplasma pneumoniae infection in children.docVIP

 PAGE \* MERGEFORMAT 3 1502 cases of respiratory tract infection analysis of mycoplasma pneumoniae infection in children Author: Shi F, Wang Ye Ping Chen Yueming [Keywords:] respiratory tract infection with Mycoplasma pneumoniae Mycoplasma pneumoniae (Mycoplasma Pneumoniae, MP) is between viruses and bacteria between a bacteria, and are caused by children, the elderly the major pathogens of respiratory tract infection, is another cause of community-acquired pneumonia is one of common diseases of the original. By random collection of respiratory tract infection in children with throat swab, using fluorescence quantitative PCR (FQ-PCR) test specimens MP content, the method has a high degree of sensitivity and specificity. Report are as follows. A clinical data 1.1 General Information January-December 2006 inter-hospital pediatric outpatient and hospitalized children, a total of 1502 cases, of which 877 cases of male and female 625 cases; aged amp;quot;15 years, an average of 4.5 years. Clinical symptoms include cough, fever, asthma and other clinical diagnosis of acute bronchitis, pneumonia, asthma, tonsillitis and myocarditis and so on. 1.2 Specimen Collection Wipe with sterile cotton swab directly to children with posterior pharyngeal, tonsil, pseudomembranous edge, the back into the tube for inspection. All specimens were handled in the day testing is completed. 1.3 MP DNA extraction With 1.5ml saline soaked cotton swabs, after repeated squeezing and dumped oscillation Cotton, the remaining liquid into the centrifuge tube, to 12000 r / min centrifugation 5 min, discard supernatant, add 50μ l DNA extract (Sun Yat-sen daan Gene Co., Ltd.) to be fully mixing, boiling water bath 10min, 12000r/min standby after 5 min centrifugation, DNA in the supernatant. 1.4 FQ-PCR amplification and results analysis The supernatant obtained by adding 2μ l PCR tube for each experiment are set positive, negative, blank control. Sample after the end Canadian ABI 7000 PC