152 cases of orthotopic small bowel transplantation in rats with surgical technical summary.docVIP

 PAGE \* MERGEFORMAT 13 152 cases of orthotopic small bowel transplantation in rats with surgical technical summary [Abstract] Objective: To summarize the rat orthotopic small bowel transplantation surgery. Methods: Male F344 and Lewis rats were used as donors and receptors. Bowel cut cover for the whole small intestine, portal vein and superior mesenteric artery. For intestinal receptors in the superior mesenteric artery and renal abdominal aorta end to side anastomosis using a continuous suture method valgus catcher for the body of the portal vein and recipient left renal vein anastomosis with Cuff tube method, removal of receptor from the small intestinal and ends for intestinal anastomosis with reconstruction of digestive tract. donor and recipient were intraoperative fluid 4 ~ 6 ml. Results: A total of 152 transplants, including 120 formal experiment, donor operation time (60 + -10) min, by body operation time (100 + -15) min, warm ischemia time of intestinal transplantation (30 + -5) min, 78.3% survived more than 2 weeks after surgery rats, pathological examination confirmed the rejection of transplanted small bowel lesions consistent characteristics. Conclusion: for bowel access, vascular anastomosis, surgery to maintain blood volume, holding a rat orthotopic small bowel transplantation in the key. [Keywords:] small bowel transplantation; rat; transplant rejection; microvascular anastomosis [Abstract] Objective: To review the surgical technique for orthotopic small bowel transplantation in rats. Methods: The male F344 and Lewis rats were donors and recipients, respectively. The entire small bowel, portol vein and superior mesenteric artery were removed in the donors. Small bowel transplantations were performed by microvacular end to side anastomosis of the donor superior mesenteric artery, and the recipient infrarenal aorta by continuous lock suture technique and by formation of a ‘cuff’ anastomosis between the donor portal vein and the recipien