2 mediated by COX-THC inhibited CA1-term depression of the role of mayor.docVIP

 PAGE \* MERGEFORMAT 14 2 mediated by COX-THC inhibited CA1-term depression of the role of mayor [Abstract] Objective To investigate cyclooxygenase-2 (cyclooxgense 2, COX 2) in the tetrahydrocannabinol (9 tetrahydrocannabinol, 9 THC) inhibition of CA1 mayor term depression (long term depression, LTD) in the role. Methods in small mice were injected intraperitoneally 9 THC (10mg/kg) or COX 2 selective inhibitor NS398 (10mg/kg) 24h after the biopsy, in hippocampal CA1 area recorded field potential EPSP (fEPSP), observe the NS398 and 9 THC’s role in the LTD combined with whole cell patch clamp technique was THC on neuronal membrane excitability. Results given low frequency stimulation (1Hz, 15min) induced CA1 area LTD, 9 THC significantly reduced LTD. 9 THC does not alter the hippocampal CA1 area basic synaptic transmission and the basic electrical properties of membrane (including membrane potential, input resistance and discharge characteristics). 9 THC significantly reduced the effect of LTD may be COX 2 selective inhibitor NS398 reversed by. In the COX 2 gene knockout mice, 9 THC reduced the role of LTD was significantly inhibited. Conclusions COX 2 in the CA1 area of the 9 THC-mediated reduction LTD role. [Keywords:] cyclooxygenase-2, THC, long-term depression; synaptic transmission, hippocampus ABSTRACT: Objective To explore the role of cyclooxgense 2 (COX 2) in tetrahydrocannabinol (THC) induced inhibition of long term depression (LTD) in CA1 area in vitro. Methods The hippocampal slices were prepared at 24h after intraperitoneal injection of 9 THC (10mg / kg) or COX 2 inhibitor NS398 (10mg/kg). Field excitatory post synaptic potentials (fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398, a selective inhibitor of COX 2, on the THC induced inhibition of LTD and THC s effect on membrane excitability in pyramidal neurons by using the whole cell patch clamp technique. Results Low frequency stimula