23-plex Y-SNPs multiplex PCR system for forensic applications of.docVIP

 PAGE \* MERGEFORMAT 18 23-plex Y-SNPs multiplex PCR system for forensic applications of Of: Aiping Liu Chao Li Yue Liu Changhui Chen Liwei, Gelu Lu, Xiao-Hui Chen [Abstract] [Objective] SNaPshot technology to build 23-plex Y-SNPs in the multiplex PCR system to study its application in forensic science. [Methods] name from the Y chromosome haplogroup tree and select the 23 Y-SNP position Points: P164, P203, P148, P145, M89, P151, M216, P128, P157, P149, P131, P199, P123, P191, P201, M111, M9, P132, P200, P197, M119, P136 and M134, the composite PCR amplification, PCR products were purified using fluorescence labeled single base extension technology (SNaPshot kit) combined with capillary electrophoresis for detection of single nucleotide polymorphisms, investigated the application of the system in Guangdong 290 unrelated men individual. [results] established 23 Y-SNPs detection system, are polymorphic loci, genetic diversity, the range of 0.013 ~ 0.491 2 7. In 290 men, 143 were detected haplotypes, haplotype diversity 0.990 7. [Conclusion] The 23 Y-SNPs detection system for fast and accurate sample SNP genotyping in forensic science, anthropology and related disciplines, the study has a high application value. [Keywords:] individual identification; single base extension (micro-sequencing); SNaPshot kit; single nucleotide polymorphism; Y chromosome Abstract: [Objective] To develope a robust single nucleotide polymorphism (SNPs) typing assay with co-amplification and to type 23 human Y chromosome SNPs using SNaPshot kit. [Methods] Markers were drawn from the phylogenetic tree of Y chromosome: P164, P203 , P148, P145, M89, P151, M216, P128, P157, P149, P131, P199, P123, P191, P201, M111, M9, P132, P200, P197, M119, P136, and M134. All 23 Y-SNPs in one reaction were amplified; the pooled PCR products were purified. The minisequencing reactions were performed simultaneously for all 23 Y-SNPs with fluorescein-labeled dideoxynucleotides. A total of 290 male