5 Dipsacus polysaccharides and dynamic distribution of.docVIP

 PAGE \* MERGEFORMAT 7 5 Dipsacus polysaccharides and dynamic distribution of [Abstract] Objective To Enshi authentic ingredients - 5 Dipsacus as raw materials, a preliminary study of its extraction and isolation of polysaccharide and its dynamic distribution. Methods petroleum ether degreasing, hot water extraction, ethanol precipitation, sevage deproteinized, freeze-drying methods such as extraction and separation of Dipsacus polysaccharide, and the use of anthrone colorimetry Dipsacus polysaccharide content, understand the dynamics of distribution of polysaccharide . Results extracted two times, feeding liquid ratio of 1:20, extraction time 2 h, extraction temperature 95 ℃ , the highest rate of Dipsacus polysaccharides, three-year-renewal of uprooting the distribution of the maximum polysaccharide. Conclusion 5 Dipsacus distribution of polysaccharides as follows: three years to take root amp;quot;for two years to take rootamp;quot; Rooting for one year amp;quot;leaves, planted continued uprootingamp;quot; Wild Root. [Keywords:] 5 Dipsacus distribution of polysaccharide extraction and isolation Abstract: ObjectiveTo extract and separate polysaccharide in Dipsacus asper and extract polysaccharide and study its regularities.MethodsDuring the extraction of polysaccharide, the effects of extraction times, temperature, proportion and time on yield of polysaccharide investigated by orthogonal experiment. The separation procedure of precipitation with alcohol and purification from the removing proteins and fats were analyzed. The contents of polysaccharides were determined by anthrone colorimetry. ResultsThe results showed that the optimum parameters were as follows: 2 times extraction ratio of material solvent 1:20, the time 2h, the temperature was 95 ℃ . ConclusionThe regularities of polysaccharide distribution in Dipsacus asper is as follows: the polysaccharide in the roots grew for three yearsamp;gt; the one for two yearsamp;gt; for one yearamp;gt; the one in