5aminolevulinic acid photodynamic therapy on human ovarian tumor cell.docVIP

 PAGE \* MERGEFORMAT 15 5aminolevulinic acid photodynamic therapy on human ovarian tumor cell [Abstract] Objective To explore 5-aminolevulinic acid (5 aminolevulinic acid, 5 ALA) mediated photodynamic therapy (photodynamic therapy, PDT) on cultured human ovarian cancer cell-killing effect of 3AO. Methods microscopy cell morphology; MTT Detection of 5 ALA-mediated PDT (5 ALA PDT) on cell proliferation 3AO; PI staining and Annexin V PI staining combined with flow cytometry 3AO 5 ALA PDT on the cell cycle and death patterns. Results after 5 ALA PDT 3AO cells showed typical morphological changes of apoptosis; cell inhibition rate increased 5 ALA concentration and the increase of laser energy density increased (P lt;0.01), when the photosensitizer concentration reaches a certain level, inhibition rate of increase is no longer appropriate, the existence of the effect of the concentration of saturation; flow cytometry showed a G0/G1 cells after PDT on the growth pause (P lt;0.05), there are obvious signs of advanced apoptosis and secondary necrosis (P lt; 0.01). 5 ALA 3AO ovarian cancer cell killing effect; 5 ALA PDT apoptosis is a mechanism of anti-ovarian cancer. [Keywords:] 5-aminolevulinic acid; photodynamic therapy; ovarian cancer; apoptosis ) ABSTRACT: Objective To study the effects of 5 aminolevulinic acid (5 ALA) mediated photodynamic therapy (PDT) on human ovarian carcinoma 3AO cells in vitro. Methods Morphological changes were examined by microscopy; the inhibitory effects of 5 aminolevulinic acid mediated photodynamic (5 ALA PDT) on 3AO cells were examined by MTT assay; cell cycle of 3AO cells was observed by flow cytometry with PI staining; the modality of 3AO cell death induced by 5 ALA PDT was determined by flow cytometry with Annexin V PI staining. Results Typical morphological changes of apoptosis occurred after 5 ALA PDT. The inhibition rate of cells increased gradually with the increase of 5 ALA concentration and laser irradiation dose (P lt